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Luciferin is a chemical substance found in the cells of various bioluminescent organisms. When Luciferin is oxidized under the catalytic effects of luciferase and ATP, light is produced. Because the reaction is dependent on ATP, it allows researchers to determine the presence of energy or life. Firefly luciferin is a particularly good reporter for in vitro biophotonic imaging due to properties of its emission spectra.
Luciferin can be used in a number of ways. It can be used in a variety of in vitro assays, where the production of light can be monitored with either a luminometer or a scintillation counter. It can also be used to monitor light production in vivo, and can be monitored with PerkinElmer's IVIS® imaging platform. Because luciferin can penetrate cell membranes, it allows transformed cells to be monitored for luciferase activity.
Luciferin can also be used with PerkinElmer's Bioware® Brite luciferase expressing oncology cell lines and our Red-FLuc lentiviral particles. There are many considerations when choosing a luciferin substrate and it is important to know that you are using the highest quality Luciferin for your experiments.
Molecular Information: C11H7 KN2 O3 S2 (MW: 318.4)
|Product Brand Name||XenoLight|
|Shipping Condition||Blue Ice|
|Unit Size||5 x 1 g|
Researchers trust our in vivo imaging solutions to give them reliable, calibrated data that reveals pathway characterization and therapeutic efficacies for a broad range of indications. Our reagents, instruments, and applications support have helped hundreds of research projects over the years. And our hard-earned expertise makes us a trusted provider of pre-clinical imaging solutions— with more than 9,000 peer reviewed articles as proof.
The primary goal of preclinical imaging is to improve the odds of clinical success and reduce drug discovery and development time and costs. Advances in non-invasive in vivo imaging techniques have raised the use of animal models in drug discovery and development to a new level by enabling quick and efficient drug screening and evaluation. Read this White Paper to learn how preclinical in vivo imaging helps to ensure that smart choices are made by providing Go/No-Go decisions and de-risking drug candidates early on, significantly reducing time to the clinic and lowering costs all while maximizing biological understanding.