The Vectra® 3 automated quantitative pathology imaging system accurately detects and measures weakly expressed and overlapping biomarkers within a single H&E, IHC or IF intact FFPE tissue section or TMA to accelerate your cancer immunology research.
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The Vectra 3 automated quantitative pathology imaging system, together with the inForm® software, provides multispectral and automated multi-slide imaging of up to 6 slides to detect and measure weakly expressed and overlapping biomarkers in H&E, IHC or IF intact FFPE tissue sections or TMAs within a familiar digital pathology workflow.
Tissue sections or TMAs can be labeled with immunofluorescent (IF) or immunohistochemical (IHC) stains such as Opal™, or with conventional stains such as H&E and trichrome. When using IF or IHC stains, multiple proteins can be measured on a per tissue, per cell, or per cell compartment (e.g. nuclear, cytoplasmic) basis - even when signals are spectrally similar, are located in the same cellular compartment or are obscured by autofluorescence.
Vectra 3 can also be used with the Phenochart™ whole slide contextual viewer with annotation capability into the digital workflow, where pathologists and technicians can navigate around slides and identify areas of interest for high-resolution multispectral acquisition. It accurately measures protein and biomarker expression and morphometric characteristics in intact tissue sections for translational studies.
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This guide provides information to configure your Vectra® 3 hardware and software to accommodate Chroma’s AQUA filter cube and the DISCOVERY fluorophores for imaging and analysis of your DISCOVERY 5-Plex immunofluorescent samples
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