check quantity

TSA Plus DNP (AP) System, for 25-75 Slides

TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.

Item(s) added to cart.

Part Number
Unit Size
List Price
Your Price
25-75 slides
289.00 USD
50-150 slides
516.00 USD
Buy Now

Please enter valid quantity

Please log in to add favorites.


Detail Information

TSA Plus DNP System for chromogenic detection with alkaline phosphatase:

  • Designed for biotin-free chromogenic detection in IHC and ISH applications
  • Eliminates background associated with endogenous biotin
  • Save time by skipping biotin quenching procedures
  • The ISH protocol included is specifically optimized for using with popular DIG-labeled probes, although any hapten-labeled probe can be used.
  • Up to 1000-fold higher sensitivity using your current antibody or probe


Detection Method Fluorescence, Chromogenic
Hapten DNP
Product Brand Name TSA Plus
Shipping Condition Blue Ice
Unit Size 25-75 slides
Resources, Events & More
  • All

Application Note

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

PDF 776 KB


TSA™ Signal Amplification for IHC, ICC, IF

The extraordinary sensitivity of the Tyramide Signal Amplification (TSA™) kits from PerkinElmer lets you see previously undetectable levels of protein and nucleic acid. The resolution is remarkable. And with multi-target detection, you can get more information from each experiment. It’s clear to see. TSA makes it easy to gain valuable insight from your immunohistochemistry (IHC) and immunocytochemistry (ICC) results.

TSA™ Signal Amplification for ISH

Tyramide Signal Amplification (TSA™) provides remarkable sensitivity enhancement for your ISH experiments without the drawbacks associated with other methods. Signal amplification with TSA allows detection at levels as low as asingle copy and enables the use of shorter probes for more precise localization of targets. The TSA reaction occurs within 10 minutes, and labels are bonded covalently, ensuring outstanding resolution.



RENAISSANCE® TSA™ Plus DNP (HRP or AP) System Manual

TSA Plus DNP technology uses HRP to catalyze the deposition of the dinitrophenyl (DNP) labeled amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins. The reaction is quick (less than 10 minutes) and results in the deposition of numerous DNP labels immediately adjacent to the immobilized HRP enzyme.

PDF 189 KB