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TSA Plus Biotin Kit, 50 - 150 slides

TSA® Plus Biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.

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Unit Size
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50-150 slides
383.00 USD
250-750 slides
1436.00 USD
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Detail Information

TSA Plus Biotin improves sensitivity in immunohistochemistry, in situ hybridization, and related experiments by up to 1000 fold while adding just 2 steps or about 20 minutes to existing protocols.




  • 10-20 times higher sensitivity than regular TSA
  • Outstanding resolution and clarity
  • Reduce non-specific background
  • Ideal for use with low affinity antibodies
  • Conserve precious reagents by using higher dilutions of primary antibodies and probe
  • Combine with labeled streptavidin for fluorescent or chromogenic detection


Detection Method Fluorescence, Chromogenic
Hapten Biotin
Product Brand Name TSA Plus
Shipping Condition Blue Ice
Unit Size 50-150 slides
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Application Note

Flow Cytometric Detection of pAKT and FoxP3 in Jurkat T Cells Using TSA™ Plus Signal Amplification Technology

Flow Cytometric Detection of pAKT and FoxP3 in Jurkat T Cells Using TSA™ Plus Signal Amplification Technology

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

PDF 776 KB


TSA™ Plus Biotin Kit Manual

TSA Plus Biotin technology uses HRP to catalyze the covalent deposition of biotin labeled amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins. The reaction is quick (less than 10 minutes) and results in the covalent deposition of numerous biotin labels immediately adjacent to the immobilized HRP enzyme. These labels can then be indirectly detected by standard chromogenic or fluorescent techniques, with significant enhancement of the signal.

PDF 380 KB