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TSA Biotin System, for 50-150 Slides

TSA® biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.

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Unit Size
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50-150 slides
319.00 USD
200-600 slides
1063.00 USD
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Detail Information

TSA Biotin Systems:

  • Signal amplification for IHC, ISH and related applications
  • Up to 1000-fold higher sensitivity
  • Conserve precious antibodies or probes
  • Flexibility in detection:
    • Chromogenic detection with streptavidin conjugated HRP or AP and substrate of choice
    • Fluorescence detection with streptavidin conjugated fluorophores
  • High resolution results
  • Adds 2 steps to existing protocol for rapid results


Detection Method Fluorescence, Chromogenic
Hapten Biotin
Product Brand Name TSA
Shipping Condition Blue Ice
Unit Size 50-150 slides
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Application Note

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

PDF 776 KB


TSA™ Signal Amplification for IHC, ICC, IF

The extraordinary sensitivity of the Tyramide Signal Amplification (TSA™) kits from PerkinElmer lets you see previously undetectable levels of protein and nucleic acid. The resolution is remarkable. And with multi-target detection, you can get more information from each experiment. It’s clear to see. TSA makes it easy to gain valuable insight from your immunohistochemistry (IHC) and immunocytochemistry (ICC) results.

TSA™ Signal Amplification for ISH

Tyramide Signal Amplification (TSA™) provides remarkable sensitivity enhancement for your ISH experiments without the drawbacks associated with other methods. Signal amplification with TSA allows detection at levels as low as asingle copy and enables the use of shorter probes for more precise localization of targets. The TSA reaction occurs within 10 minutes, and labels are bonded covalently, ensuring outstanding resolution.



TSA™ Biotin Kits Manual

The TSA Biotin System technology uses HRP to catalyze the deposition of the biotin-labeled tyramide (amplification reagent) onto tissue sections or cell preparation surfaces that have been previously blocked with proteins. The reaction is quick (less than 10 minutes) and results in the deposition of numerous biotin labels immediately adjacent to the immobilized HRP enzyme.

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