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Spotlight 400N FT-NIR Imaging System

Spotlight IR microscope systems are designed to meet the challenges of an expanding laboratory by generating high-quality, reproducible data from a variety of sample types. The Spotlight 400N FT-NIR Imaging System combines high sensitivity and rapid imaging with ease-of-use. The ability to image large sample areas rapidly at high spatial resolution extends FT-NIR microscopy into new applications.

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FT-NIR Imaging for Exceptional Analyses

The Spotlight 400N FT-NIR Imaging System is designed with state-of-the-art technology to allow for intelligent automation and sophisticated analysis capabilities. The system incorporates a number of unique productivity tools and features an ATR imaging system that enables the collection of high resolution infrared images of extremely small samples.

Unique features of the Spotlight 400N System include:

  • High quality spectral production and images from sample areas, yielding pixel resolutions of 6.25, 25, or 50 microns
  • Region of Interest (ROI) finding to allow for ease of analysis of multiple particles and layers all at once
  • Configurability to be used with extended range mid-IR, near-IR or dual range FT-IR with the Frontier™ system to give maximum information from samples in the shortest possible time.
  • Configurability to be used with mid-IR, near-IR, or dual range FT-IR with the Frontier™ or Spectrum Two™ systems to give maximum information from samples in the shortest possible time.

The Spotlight 400N FT-NIR Imaging System can be configured to meet your FT-NIR microscopy demands and produce high-quality spectra and FT-NIR images from extremely small samples. See the Configurations table below for standard options, and contact a sales representative for more information to customize a system to suit your analysis needs.

  • Standard InGaAs (indium gallium arsenide) point and array detector for high-performance near-IR applications
  • Micro-ATR objective with a choice of a silicon or germanium crystal
  • ATR imaging objective with a germanium crystal with automatic switching between wide area survey and high definition imaging modes
  • Standard motorized stage that can be controlled by a joystick or through Spectrum software
  • Standard motorized stage that can be controlled by a joystick or through Spectrum software
  • White light LED illumination for true color display with capabilities for auto-illumination and autofocus modes
  • Spectrum 10™ software as standard with an option for enhanced security in Spectrum 10 ES™
  • SpectrumIMAGE™ software as standard enabling full operation over the imaging system
  • Can be configured as a system with Spectrum Two or purchased as an upgrade package to an existing PerkinElmer FT-NIR spectrometer.

Microscopy for a Variety of Applications

Easy to use, yet exceptionally powerful and versatile, the Spotlight’s flexibility and sensitivity make it a perfect addition to any lab setting, in any discipline. Some of the applications supported by the Spotlight 400N System include:

  • Materials Analysis
  • Forensics
  • Pharmaceuticals
  • Biomedical Research
  • Academic Research
  • Biomaterials

Results Matter

The Spectrum 10 software suite drives the Spotlight 400N system and can support advanced FT-NIR analysis in research and industrial laboratories, always providing optimal results. The software supplied with the system gives you full control over the microscope including the focusing on the sample, illumination, stage position, changing between sampling modes, and manipulating the spectra that you collect. The spectrometers can operate in ratio, single-beam, or interferogram mode. SpectrumIMAGE software enables control over the imaging system with the ability to view sample images with ease in real time. With Spectrum MultiSearch, identification of unknown mixtures is made simple through the use of comparison algorithms allowing for the separation and library matching of up to 10 components.

For regulated industries that demand secure IR quality testing, Spectrum 10 ES™ software is available and encompasses user access control, audit trailing, data security, and data integrity to ensure secure analyses to meet the technical demands of 21 CFR Part 11 compliance.


21 CFR Part 11 Compatible Yes
Depth 57.0 cm
Detector Type InGaAs
Height 47.0 cm
Operating Range 5 – 40 °C
Portable No
Product Brand Name Spotlight
Product Group Instrument
Technology Type Infrared
Warranty 1 year
Wave Length 10
Wave Length Range 7,800 – 4,000 cm-1
Weight 32.0 kg
Width 34.0 cm


Model Spotlight Detector Frontier Options Standard Features Optional Extras
Spotlight 400N – Frontier NIR MCT or InGaAs
  • Standard NIR system with DTGS detector
  • Spectrum 10
  • SpectrumIMAGE
  • MultiSearch
  • Motorized stage control unit
  • PC with Windows 7 and stage controller
  • AutoATR objective
  • ATR Imaging Accessory
  • NIR reflectance accessory
  • Microscopy polarizer kit
  • Heated stage accessory
  • Micro-sampling tool kit
Spotlight 400 – Frontier MIR/NIR InGaAs
  • System with MIR and NIR DTGS detectors

Resources, Events & More

All (20)
Resource Type File Name File Format
Brochure Infrared Imaging and Microscopy Systems PDF  7 MB
Brochure Spotlight™ 400 FT-IR and 400N FT-NIR Imaging Systems PDF  7 MB
Brochure Spotlight™ 400 FT-IR and 400N FT-NIR Imaging Systems Brochure PDF  1 MB
Product Note Variable Temperature Stage Options for FT-IR Microscopes and Imaging Systems PDF  255 KB
Guide Material Characterization Instrument Guide PDF  6 MB
Guide Solutions and Services For Better Results PDF  5 MB
Application Note ATR FT-IR Imaging of Human Hair Cross-Section PDF  598 KB
Application Note ATR Imaging of Pharmaceutical Tablets PDF  1 MB
Application Note Augmenting Standard Methods of Measuring Airborne PM2.5 Using IR Imaging PDF  1 MB
Application Note FT-IR Imaging Dara from Sectioned Tomato Seed PDF  346 KB
Application Note FT-IR Imaging Study on Stoma of Limonium PDF  610 KB
Application Note IR Microscopy Used in Effort to Solve Mystery: Did Mallory Climb Everest in 1924? PDF  175 KB
Application Note Measurement of Counterfeit Pharmaceuticals Using the Spotlight Imaging System PDF  1 MB
Technical Note Large Area ATR FT-IR Imaging Using the Spotlight FT-IR Imaging System PDF  702 KB
Technical Note Spatial Resolution in ATR FT-IR Imaging: Measurement and Interpretation PDF  264 KB
Poster Tablet uniformity measurements using Near-IR Spectroscopy PDF  308 KB
Case Study Infrared Microscopy Provides More Accurate Kidney Stone Diagnosis PDF  436 KB
Case Study Spotlight FT-IR Imaging System Helps Forensic Lab Win Triple Murder Conviction PDF  306 KB
Product Info Polymer Applications Compendium PDF  11 MB
Regulatory Compliance Certification Spotlight 400 PDF  63 KB