Spotlight™ IR microscope systems are designed to meet the challenges of an expanding laboratory by generating high-quality, reproducible data from a variety of sample types. The Spotlight 400 FT-IR Imaging System combines high sensitivity and rapid imaging with ease-of-use. The ability to image large sample areas rapidly at high spatial resolution extends FT-IR microscopy into new applications.
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FT-IR Imaging for Exceptional Analyses
The Spotlight 400 FT-IR Imaging System is designed with state-of-the-art technology to allow for intelligent automation and sophisticated analysis capabilities. The system incorporates a number of unique productivity tools and features an ATR imaging system that enables the collection of high resolution infrared images of extremely small samples to visualize the composition of materials based on FT-IR spectral data.
Unique features of the Spotlight 400 System include:
The Spotlight 400 FT-IR Imaging System can be configured to meet your FT-IR microscopy demands and produce high-quality spectra and FT-IR images from extremely small samples. See the Configurations table below for standard options, and contact a sales representative for more information to customize a system to suit your analysis needs.
A variety of optional accessories and consumables are available for use with the Spotlight systems, such as an infrared polarizer kit, heated stage accessory, crystal compression cells, and a micro-sampling tool kit for ease with sample handling and preparation.
Microscopy for a Variety of Applications
Easy to use, yet exceptionally powerful and versatile, the Spotlight’s flexibility and sensitivity make it a perfect addition to any lab setting, in any discipline. Some of the applications supported by the Spotlight 400 System include:
The powerful software suite drives the Spotlight 400 system and can support advanced FT-IR analysis in research and industrial laboratories, always providing optimal results. The software supplied with the system gives you full control over the microscope including the focusing on the sample, illumination, stage position, changing between sampling modes, and manipulating the spectra that you collect. The spectrometers can operate in ratio, single-beam, or interferogram mode. SpectrumIMAGE software enables control over the imaging system with the ability to view sample images with ease in real time. With Spectrum MultiSearch, identification of unknown mixtures is made simple through the use of comparison algorithms allowing for the separation and library matching of up to 10 components.
|21 CFR Part 11 Compatible||Yes|
|Operating Range||5 – 40 °C|
|Product Brand Name||Spotlight|
|Wave Length||7800-600 cm-1|
|Wave Length Range||7,800 – 600 cm-1|
|Model||Spotlight Detector||Frontier Options||Standard Features||Optional Extras|
|Spotlight 400 – Frontier MIR||MCT or DTGS||
|Spotlight 400 – Frontier MIR/NIR||MCT or DTGS||
|Spotlight 400 – Frontier MIR/FIR||MCT or DTGS||
Human hair is typically 50-100 µ in diameter, previous transmission and ATR studies of its cross section1,2 reveal three major identifiable regions.
Technology improvements and increased use has established chemical imaging as a means of providing information on certain critical quality parameters that is not readily addressable using other techniques.
The use of IR imaging expands the measurement possibilities by giving information about the individual types of materials present in the collected particles. The information from the IR imaging experiment is not only qualitative (i.e. identification of the particulates), but can also be calibrated to give quantitative information on the components present. The IR imaging measurement will take only five minutes, whereas Ion Chromatography requires solvent extraction of the particulates and would take around 20-25 minutes per sample
FT-IR Imaging has excellent potential in sample characterization within the Injectables segment of Pharma and Biopharma sector for impurity identification.
The following data was obtained from a sectioned of tomato seed and analyzed using the PerkinElmer spotlight FT-IR imaging system. The section was placed onto a Barium Fluoride window and the infrared data was obtained in transmission mode.
There are many stomata (thousands per square centimeter) in the lower epidermis of plant leaves. Two crescent-shaped cells called guard cells, which are rich in chloroplasts, flank the stoma. Starch is produced in daytime by photosynthesis within the guard cells, not in adjacent epidermal cells.
Bottled water is marketed as being the clean and pure alternative to drinking tap water. However, there is increasing opposition to the use of single-use plastic bottles and the fact that they end up in the environment either as intact bottles, or as secondary microplastics when they do break down into smaller pieces and particles. A recent study also reports that microplastic particles have been detected in several brands of bottled drinking water. The effects on human health from microplastics are still to be determined, but the presence of microplastics, potentially containing priority organic pollutants (POPs), in food and beverages is a major concern. Infrared (IR) spectroscopy is the primary analytical technique for the identification of polymers and the use of IR microscopy allows for the detection and identification of microplastics down to particles of only a few microns in size. This application note describes the analysis of several different brands of bottled water using the PerkinElmer Spotlight 400 FT-IR Imaging system.
FT-IR Microscopy quickly provides a unique fingerprint of a complicated material made up of multiple constituents. Another advantage of this technique is that it requires little or no sample preparation and that it can generate spectra in a matter of a few minutes.
The Spotlight FT-NIR Imaging System is ideally suited to the rapid identification of counterfeit drugs based on the distribution of ingredients.
Introducing the PerkinElmer infrared imaging and microscopy systems family.
Choose the PerkinElmer Frontier™ range of near-, mid- and far-IR Fourier Transform spectrometers for superior spectroscopic performance in demanding applications. Powerful and adaptable, the Frontier meets all your current analysis needs and can be expanded as your research goals evolve. And with automated range switching, mid- near- or far-IR techniques are available at your fingertips. An exceptional signal-to-noise ratio and photometric performance assures optimal spectral performance to ensure best-in-class sensitivity. This configurable platform provides dependable, consistent and trouble-free operation through years of service.
Frontier™ is PerkinElmer’s most powerful, adaptable IR solution yet, specially developed to offer superior performance in demanding applications.
With Spotlight 400 FT-IR and Spotlight 400N FT-NIR imaging systems, you experience unprecedented, uncompromising data quality and clear, complete, highly detailed results from all your samples. Spotlight FT-IR systems are purpose-built for a wide range of demanding imaging applications. So you’re able to switch between sampling modes – standard transmission, reflection, ATR imaging, and more – with ease, and your images can be collected.
It’s not every day that a masterpiece is created. But how else does one describe a laboratory instrument that will dramatically improve the understanding of materials across an unprecedented range of industries. With the Spotlight™ 400, IR imaging just became faster, more efficient and more flexible than ever before. It is quite simply the most productive laboratory FT-IR imaging system in the world.
A recent study has demonstrated that the accuracy of kidney stone diagnosis can be substantially improved by switching from conventional optical microscopy and contrast staining to infrared (IR) microscopy. There are over a dozen different types of kidney stone, and diagnosing the type is important because most effective treatment methods vary for each.
Analyses using infrared microspectroscopy; performed by the Michigan State Police Forensic; Science Division (MSPFSD); Lansing Laboratory; helped to win a triple murder conviction by matching paint from one victim’s clothing to the murderer’s car.
Guide describing all UV/Vis spectroscopy, FT-IR spectroscopy, thermal analysis, hyphenation technique solutions for material characterization.
Poster summarizing solutions of thermal analysis, molecular spectroscopy, chromatography and hyphenated techniques for polymers focused on providing more insight into product performance and process optimization that make easier
This poster describes analytical application example of FT-IR microspectroscopy for microplastic analysis which are used as exfoliating microbeads in cosmetics and personal care products
Among the FDA’s top reasons for product recall were problems with sub-potency and tablet dissolution. In addition, recent cases of cGMP violations have highlighted issues in tablet manufacturing. One area where industry is seeking to improve its processes is in the blending area.
Using spectrum touch software and predefined operating procedures in IR and NIR analyses can help fulfill regulatory compliance requirements, decrease error rate and enable better communication of effective analysis with stakeholders
Product note dscribes TIBCO Spotfire for IR Instrumentation software product from PerkinElmer that creates powerful visualizations to ensure the quality and consistency of your results-every time.
There is an increased interest in the microscopic analysis of samples using FT-IR variable-temperature microscopy or imaging. Application areas include polymer studies, pharmaceutical and food analysis and geological measurements. PerkinElmer offers two options for variable temperature analyses – Linkam variable temperature sample cell and the hot stage accessory.
ATR FT-IR imaging offers users easy spectroscopic access to “difficult” samples which are hard to image in transmission or reflection, or which have confusing depth structure.
ATR FT-IR imaging with germanium crystals offers the potential for acquiring infrared spectral images at up to four times higher spatial resolution than is possible using conventional reflectance or transmission imaging.
This white paper explains the problem of microplastics in water and what are the best instruments to trace, identify, and classify microbeads in products, food, and the environment.