PerkinElmer
check quantity

Alpha SureFire® Ultra™ Multiplex acetyl/total p53 Assay Kit, 50,000 Assay Points

The Alpha SureFire® Ultra™ Multiplex p53 (K382Ac) + Total p53 assay kit is used to measure both the acetylation (Lys382) and total levels of endogenous p53 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p53 acetylation by either recombinant or endogenous receptors, and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
MPSU-ATP53-K-HV
100 Assay Points
946.00 USD
 
more
MPSU-ATP53-K500
500 Assay Points
3197.00 USD
 
more
MPSU-ATP53-K10K
10,000 Assay Points
19300.00 USD
 
more
MPSU-ATP53-K50K
50,000 Assay Points
60600.00 USD
 
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

The Alpha SureFire Ultra Multiplex p-p53 + Total p53 assay kit is used to measure both phosphorylated and total levels of endogenous p53 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated p53 analysis, and the 545 nm (Tb) signal corresponds to the total p53 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Target p53
Assay Target Class Acetylated protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name Alpha SureFire Ultra Multiplex
Shipping Condition Blue Ice
Unit Size 50,000 Assay Points
Resources, Events & More
  • All

Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.

PDF 1 MB