The Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells.
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For research use only. Not for use in diagnostic procedures.
The Alpha SureFire Ultra HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells.
The 615nm (Eu) signal corresponds to the phosphorylated ERK analysis, and the 545nm (Tb) signal corresponds to the total ERK analysis.
This kit has been formulated to provide improved signal:background (i.e. S:B) assay windows, and to perform without interference in the presence of extraneous antibodies.
In this assay, the Alpha 615 Acceptor bead is directly-conjugated with an antibody to the phosphorylated site on the target protein. The Alpha 545 Acceptor bead is coated with the CaptSure agent, which binds the CaptSure tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
The antibodies used in this kit are identical to those used in the individual phospho- and total- ERK AlphaLISA SureFire Ultra kits (ALSU-PERK-A and ALSU-TERK-A catalog numbers), although their orientation of bead attachment may vary between kits.
| Assay Pathway | MAPK |
|---|---|
| Assay Target | ERK1/2 |
| Assay Target Class | Phosphoprotein, Protein |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Molecular Modification | Phosphorylation |
| Product Brand Name | Alpha SureFire Ultra Multiplex, AlphaPlex |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Unit Size | 500 assay points |
