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Alpha SureFire® Ultra Multiplex p-AKT (Ser473) (directly coupled) HV Kit, 100 Assay Points

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).

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Part Number Unit Size List Price Your Price Quantity
MPSU-PAKT-E-HV 100 Assay Points 475.00 USD
MPSU-PAKT-E500 500 Assay Points 1605.00 USD
MPSU-PAKT-E10K 10,000 Assay Points 8800.00 USD
MPSU-PAKT-E50K 50,000 Assay Points 27600.00 USD
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Overview

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits.

This kit is designed to be used with any other AlphaLISA SureFire Ultra assay kit to allow multiplexed measurement of pAKT1/2/3 with the target of another AlphaLISA SureFire Ultra kit (“Target 2”). The “Alpha 615 anti-p-AKT1/2/3 (mIgG1) Acceptor Beads” in this kit are directly-conjugated with an antibody to the phosphorylated site on p-AKT and, in combination with the biotinylated antibody provided in this kit, will generate a 615 nm signal relative to the levels of p-AKT. The “Alpha 545 CaptSure Acceptor Beads” are coated with the CaptSure agent, which binds CaptSure-tagged antibodies, and are used in place of the “AlphaLISA® CaptSure Acceptor Beads” from the AlphaLISA SureFire Ultra kit. The use of the CaptSure beads from this kit will thus be shifting the wavelength at which the Target 2 will be read (i.e. Target 2, from the AlphaLISA SureFire Ultra kit, will now be read at 545 nm instead of the initial 615 nm in the AlphaLISA SureFire Ultra format).

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target Akt1/2/3
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Phosphorylation
Product Brand Name Alpha SureFire Ultra Multiplex, AlphaPlex
Shipping Condition Blue Ice
Unit Size 100 Assay Points