Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
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This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
AequoScreen® Double Transfected Cell Line: Rat Mas-related MrgB3 receptor in aequorin CHO-K1 host cell. We provide two vials of cryopreserved cells (approximately 2.5x106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant channel in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
Features:
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
| Assay Target Class | GPCR |
|---|---|
| Assay Target Type | Cell line |
| Assay Validated | Calcium Luminescence |
| G-Alpha Natural Receptor | Gq/G11 |
| Host Cell | CHO-K1 |
| Product Brand Name | AequoScreen |
| Receptor Type | Mas related |
| Second Messenger Release | Calcium flux |
| Shipping Condition | Dry Ice |
| Unit Size | 5 million cells |
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.
PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance
