Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
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This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
AequoScreen® Double Transfected Cell Lines: Prokineticin, PKR1 subtype. Human Recombinant, in CHO-K1 host cell. Two vials of cryopreserved cells are shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
|Assay Target Class||GPCR|
|Assay Target Type||Cell line|
|Assay Validated||Binding, Calcium Luminescence, Calcium Fluorescence|
|G-Alpha Coupling Protein||Ga16|
|G-Alpha Natural Receptor||Gq/G11|
|Product Brand Name||AequoScreen|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Unit Size||5 million cells|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Now you can characterize cellular signaling mechanisms and better understand the complexity of multiple signaling pathways more efficiently. Only the EnSpire® Multimode Plate Reader offers established Corning® Epic® label-free technology in addition to labeled technologies all within one flexible benchtop reader. This exclusive cellular and biochemical label-free platform, in conjunction with labeled technologies, provides a unique orthogonal approach, enabling you to make better decisions faster.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance
We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR