1536-well poly-D-lysine-coated (PDL-coated), tissue culture-treated (TC-treated) sterile microplate with black well walls and an optically clear bottom for cell-based assays.
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PerkinElmer's ViewPlate Microplates are engineered for bottom reading systems and microscopic viewing of cells. This high quality microplate can be utilized in your cell based assay for superior results
Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As Poly-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants. Almost all cell types will adhere to Poly-lysine coated plate bottoms.
Leveraging years of assay and instrument experience in plate detection, PerkinElmer designs better Microplates for better performance that guarantees better results for all PerkinElmer applications.
|Detection Method||Confocal Imaging, Time-Resolved Fluorescence (TRF), Luminescence, Fluorescence, High Content Imaging, Radiometric|
|Product Brand Name||ViewPlate|
|Unit Size||Case of 10|
|Wells Number||1536 well plate|
We have shown here that signal intensity for the selected catalog aequorin cell lines is strong enough to allow its measurement by the non-luminescence dedicated FLIPR