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Opal Polaris 7 Color Automation IHC Detection Kit

Introducing Phenoptics 2.0: Opal Polaris Kits are part of a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, across the entire digital slide. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed. Opal Polaris 7-Color IHC Detection Kits are optimized for whole slide multispectral imaging with the Vectra® Polaris Automated Quantitative Pathology Imaging Instrument.

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For research use only. Not for use in diagnostic procedures.

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Detail Information

The Opal Polaris 7-Color Automation IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:

  • 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
  • Spectral DAPI
  • 1X Plus Automation Amplification Diluent
  • Blocking/Ab Diluent
  • Opal Polymer HRP Ms + Rb
  • Users should source primary antibodies and other workflow reagents separately. The Opal Polaris 7-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® Polaris system. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.

Opal will enable you to:

  • Measure up to six tissue biomarkers at once
  • Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
  • Retain spatial cellular context that is lost in flow cytometry
  • Confirm single cell co-expression for many biomarkers in one tissue section
  • Get more information while conserving precious tissue.

Automating Opal staining brings the benefit of:

  • Quality, consistency and reproducibility with every sample
  • Time-savings versus laborious manual process
  • Ease-of-use with a walk-away, high-throughput protocol


Automation Compatible Yes
Detection Method MSI, Fluorescence
Fluorescent Agent Type Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780
Product Brand Name Opal
Quantity in a Package Amount 1.0 per pack
Sample Type Human FFPE
Shipping Condition Blue Ice
Species Reactivity Rabbit and Mouse Primary Antibodies
Unit Size 50 slides
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Product Note

Product Note: Mantra Quantitative Pathology Workstation

The Mantra™ quantitative pathology workstation, with inForm® image analysis software, enables easy visualization, quantification, and phenotyping of multiple types of immune cells simultaneously in intact FFPE tissue sections for cancer immunology research.