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Opal 4 Lymphocyte Kit

The Opal 4 Tumor Lymphocyte Kit enables phenotyping of helper T cells, cytotoxic T cells and B cells within intact FFPE tissue sections.

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For research use only. Not for use in diagnostic procedures

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Detail Information

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, similar to standard immunohistochemistry (IHC).

The Opal 4 Tumor Lymphocyte Kit includes all critical workflow reagents to enable phenotyping of helper T cells, cytotoxic T cells and B cells within intact FFPE tissue sections with 3-plex fluorescent immunohistochemistry:

  • Primary antibodies
  • Antibody diluent
  • Fluorescent IHC detection reagents
  • Spectral DAPI
  • Antigen retrieval buffers

This kit was validated for multispectral imaging on the Vectra and Mantra Systems and should also provide results with fluorescence microscopes that include emission filters for DAPI, FITC, CY3 and CY5. It contains enough reagents for 3-plex immunostain of 50 slides.

  • Optimized panel for assessment of immune cells in intact FFPE tissue
  • Shortest path to results
  • Part of the Phenoptics workflow for immunophenotyping of solid tumors


Assay Target CD4,CD8,CD20
Automation Compatible No
Detection Method MSI, Fluorescence
Fluorophore Opal 520,Opal 570,Opal 670
Product Brand Name Opal
Quantity in a Package Amount 1.0 per pack
Shipping Condition Blue Ice
Target Species Human
Unit Size 50 slides
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