PerkinElmer
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NEXTFLEX® PCR-Free DNA-Seq Kit 8 rxn

The NEXTFLEX® PCR-Free DNA Library Prep kit completely eliminates the need for amplification, decreasing duplicate sequences, for more representative base identities and better de novo assembly.

For research use only. Not for use in diagnostic procedures.

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NOVA-5142-01
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Overview

The NEXTFLEX® PCR-Free DNA Sequencing kit is designed to prepare gel-free libraries using magnetic bead-based size selection from as little as 500 ng of input DNA. Amplifying AT or GC rich genomic regions often leads to sequence biased nucleotide compositions and poses a serious challenge during analysis. Using specially designed master-mixed enzymes, the NEXTFLEX® PCR-Free DNA Library Prep Kit completely eliminates the need for amplification, enabling better read mapping and a reduction in duplicate sequences, leading to reduced sequence cost and bias.

Learn more about the NEXTFLEX® PCR-Free DNA Library Prep kit

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