The PerkinElmer LC 300 platform redefines the liquid chromatography experience, offering the flexibility, performance and efficiency needed to tackle even the most challenging analytical demands. With the option to customize your experience with a wide range of detectors and accessories, each LC 300 system is designed to meet the most important specifications – yours. Paired with SimplicityChrom™ CDS software, the LC 300 UHPLC system delivers an elevated user experience with simplified liquid chromatography workflows, intuitive functions, and automated processes.
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The PerkinElmer LC 300 UHPLC system has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the highly intuitive and powerful SimplicityChrom™ chromatography data system (CDS) software, our LC 300 systems are the ideal solution for labs seeking to maximize their liquid chromatography experience.
The LC 300 offers superior performance and flexibility throughout its intelligent design:
|Height||38.2 inches maximum (single stack with detector and standalone solvent organizer)|
|Weight||183 pounds (single stack with one detector and standalone solvent organizer) or 260 pounds maximum (dual stack with external oven)|
|Width||13.5 inches maximum per component|
Lonicera Japonica Flos is a Traditional Chinese Medicine (TCM) used for the treatment of inflammation and infectious diseases. Chinese Pharmacopoeia has developed a method to support quality control efforts which includes the analysis of seven components in Lonicera Japonica Flos samples.
In this application brief, multiple marker compounds, including chlorogenic acid (CA), based on Chinese Pharmacopoeia standards, were analyzed using a PerkinElmer LC 300 UHPLC system, PerkinElmer Epic™ C18 column, a variety of chromatography consumables, and SimplicityChrom™ software.
Utilized for throat inflammation in children, Xiaoer Yanbian Keli granules are a traditional Chinese formulated medicine, comprised of the Lonicera Japonica herbal plant. Chinese Pharmacopoeia developed standards in 2020 to ensure quality control, and has finalized the assay of a method in which the specification of the assay of chlorogenic acid is not less than 3 mg per bag.
In this application brief, quantification of the marker compound chlorogenic acid (CA) in a formulated drug, based on Chinese Pharmacopoeia 2020, is presented using a PerkinElmer LC 300 UHPLC with SimplicityChrom™ software, PerkinElmer Epic™ C18 column, and additional chromatography consumables.
Acetaminophen, aspirin and caffeine used in combination are an effective method of treating acute headaches and migraines. The combination of these substances is considered superior to acetaminophen on its own when treating headaches. There are other benefits due to the individual components of the tablets. Acetaminophen (also known as paracetamol) is the most common analgesic and antipyretic around the world, it is an effective treatment for acute primary headaches on its own and can be combined with tramadol to treat cluster headaches. It has a very low risk of causing allergic reactions and so can be used by those with bronchial asthma. Aspirin is a first-line therapy to treat moderate to severe primary headaches as well as reducing pain, fever and inflammation. However, it is not taken by children under twelve due to the risk of Reye syndrome. Caffeine is considered an effective treatment for various types of headaches including post-dural puncture headaches and hypnic headaches. It also provides an increase in high energy exercise tolerance and reduces fatigue. These drugs combined are commonly sold under the brand names Anadin Extra in the UK and Excedrin in the U.S. In 2019 approximately 13.1 million units of Excedrin Migraine were sold in the United States. This application brief describes the use of an ES Industries Epic C18 column for the analysis of acetaminophen, aspirin and caffeine in accordance with the official Acetaminophen, Aspirin and Caffeine Tablets United States Pharmacopeia (USP) method for the assay.
Amlodipine besylate belongs to a class of drugs known as dihydropyridine calcium channel blockers. It is primarily used to treat hypertension and angina as well as other conditions which arise as a result of coronary artery disease. As a calcium channel blocker, it prevents diffusion of calcium into cardiac muscle and the smooth muscle of the blood vessels. This causes vasodilation, lower heart contractility and therefore lower blood pressure. In addition, production of nitric oxide (a known vasodilator) increases. Amlodipine is listed on the World Health Organization’s (WHO) list of essential medicines as an antihypertensive medicine, meaning that it considered among the safest and most effective compounds needed to meet the requirements of a basic healthcare system. This application brief describes the use of our Epic C18 column to analyze amlodipine besylate in accordance with the official USP monograph.
Glucocorticoid steroids work by suppressing various aspects of the human immune system in conditions where hyperactivity can cause poor health through allergies, inflammation, and autoimmune dysfunction. Betamethasone dipropionate belongs to this class of steroids. It is used for its high potency as an anti-inflammatory and immunosuppressant in the treatment of diseases such as eczema, dermatitis, and psoriasis. Betamethasone dipropionate is classified as a ‘super-potent’ steroid in the treatment of psoriasis in comparison with betamethasone valerate (another common analogue of betamethasone) which is rated as upper mid-strength. This application brief describes the use of an Epic C18 column for the analysis of betamethasone dipropionate in accordance with the official USP monograph.
The term ginseng can refer to several natural products and herbal remedies usually produced from the plant, panax ginseng. Panax ginseng is considered a traditional Chinese medicine (TCM) as it has a reported wide range of medicinal properties such as antioxidant effects and promotion of immune function.1 The chemistry of ginseng is still not fully understood and more research is required to understand its functions as it becomes increasingly popular in western culture. The main active components of ginseng are ginsenosides of which there are over 100 in various forms of panax ginseng. Ginsenosides are named as Ginsenoside Rx, with x representing the movement on a thin layer chromatography plate, x represents a letter from a-h with a being the most polar and h being the least. This application brief describes the use of an Epic C18 to analyze three ginsenosides using a method based on the Chinese Pharmacopeia (ChP).
Finasteride is an antiandrogenic compound or ‘testosterone blocker’ which is used as a treatment for an enlarged prostate and can treat male hair loss. It inhibits the production of the enzyme Type-II 5a-reductase which converts testosterone into the more active metabolite 5a-dihydrotestosterone (DHT). DHT is responsible for the development and enlargement of the prostate and can contribute to hair loss by shortening hair follicles and causing them to fall out more quickly. By suppressing production of DHT, finasteride directly targets the molecule responsible for stimulation of prostatic growth. Finasteride is commonly sold under the brand name Proscar and in 2016 was the 75th most prescribed drug in the USA. This application brief describes the use of an Epic C18 column to analyze finasteride in accordance with the official Finasteride Tablets USP monograph.
Hydrocortisone is the pharmaceutical term for cortisol used in oral administration, intravenous injection, or topical application. It is often used in the treatment of severe allergic reactions, such as anaphylaxis and angioedema. United States Pharmacopeia (USP) provides monographs for the identification and impurity testing of the drug substances. This application brief describes the assay analysis of hydrocortisone in accordance with USP monographs using a PerkinElmer LC 300 liquid chromatography system with SimplicityChrom™ chromatography data system (CDS) and PerkinElmer Epic™ columns.
Lamotrigine is an antiepileptic drug belonging in the phenyl triazine class. It is used in the treatment of both epilepsy and as a mood stabilizer for bipolar disorder. Lamotrigine related compound B also known as 2,3-dichlorobenzoic acid is a key intermediate for the antiepileptic drug Lamotrigine. It may be in found in the final product and needs to be monitored and controlled over the shelf life of the product.
The methods for assessing Lamotrigine related compound B are described in the United States Pharmacopeia1 and rely on chromatographic techniques. This application brief describes use of an Epic C18 column coupled with the LC 300 liquid chromatography system and SimplicityChrom chromatography data system (CDS).
Azithromycin, discovered in 1980, is a semi-synthetic macrolide antibiotic related to erythromycin. The drug is widely used to treat chest infections such as pneumonia, infections of the nose and throat such as sinus infection (sinusitis), skin infections, Lyme disease, and some sexually transmitted infections. The mode of action of azithromycin is like other macrolide antibiotics, it inhibits bacterial protein synthesis by binding to and interfering with the assembly of the 50S large ribosomal subunit of the bacteria, thereby inhibiting translation of mRNA. This application brief describes the use of an Epic C18 column for the simple analysis of azithromycin.
Chaenomeles, the fruit of the Chinese flowering quince, is utilized in Traditional Chinese Medicine (TCM) for its analgesic, anti-inflammatory, antispasmodic and astringent properties, and has also been shown to aid in digestion. For implementation of quality control processes, Chinese Pharmacopoeia has released the assay of oleanolic acid and ursonic acid of the herbal plant. In this work, the analysis of these two compounds, following Chinese Pharmacopoeia 2020 standards, is presented utilizing a PerkinElmer LC 300 system with SimplicityChrom™ software, PerkinElmer Epic™ C18 column and additional chromatography consumables.
Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chromatography methods for the determination of cannabinoid content are need to satisfy both regulatory and consumer demands.
In this application note, a fast and simple method for the determination of 16 common cannabinoids is presented. Utilizing a PerkinElmer LC 300 HPLC system with a PDA detector, the method results in the elution of all sixteen compounds in under seven minutes, with LOQs = 0.15 µg/mL for most analytes.
Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exhibit weak endocrine disrupting effects on users of the products, an effect that can be further intensified by the ability of parabens to be absorbed by the skin without being broken done by esterase enzymes.
In this application note, a fast, robust and sensitive method utilizing a PerkinElmer LC 300 HPLC system with Multi-Wavelength UV/Vis (MWD) detector for the analysis of 2-phenoxyethanol and seven commonly used parabens in eight different commercially available cosmetic and personal care products is presented.
Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the development of certain cancers, as well as an improvement in cholesterol ratios. Studies also suggest, however, that the phytoestrogenic nature of isoflavones can also lead to an exacerbation of existing thyroid disorders, owing to a disruption to the endocrine system. As such, precise quantification of isoflavone content in nutraceutical products is paramount to ensure label-claim accuracy and consumer safety.
In this application note, an efficient and reliable method for the determination of six common isoflavone compounds is presented. The method, which was developed in accordance with the USP monograph for soy isoflavones in dietary supplement capsules, utilizes a PerkinElmer LC 300 UHPLC system, with subsequent detection of compounds achieved utilizing an LC 300 PDA detector.
High fructose corn syrup (HFCS) as a substitute for sucrose in a wide variety of products, including baked goods, sauces, beverages, canned fruits and dairy products. HFCS is produced from regular corn syrup, with an enzymatic process that isomerizes some of the corn syrup's glucose into fructose. The fructose concentration can vary, depending upon the intended use, and is commonly produced at concentrations of 42% (HFCS-42), 55% (HFCS-55) and 90% (HFCS-90).
The US Food and Drug Administration Code of Federal Regulations have incorporated identity standards for the sugar composition of HFCS, specifically around fructose content where HFCS-42 cannot be less than 42% fructose and HFCS-55 cannot be less than 55% fructose. This application note demonstrates the suitability of the PerkinElmer LC 300 HPLC system for performing saccharide content determination via method CRA SACCH.03 through the analysis of nine commercially available high fructose corn syrups.
Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a complete identification and quantification of the other complex carbohydrates, including glucose, fructose and maltose, is essential.
In this application note, a robust and efficient method for the determination of the four aforementioned complex carbohydrates is presented. Utilizing an LC 300 HPLC system, a hydrophilic interaction chromatography (HILIC) technique is utilized with subsequent detection achieved utilizing an LC 300 Refractive Index (RI) detector.
Lamotrigine is an antiepileptic drug in the phenyl triazine class used in the treatment of both epilepsy and as a mood stabilizer for bipolar disorder. It is the first medication since lithium to be granted US FDA approval for maintenance treatment of bipolar type 1, and is approved for use in more than 30 countries.
In this work, the analysis of Lamotrigine, in accordance with the USP monograph, is detailed. Utilizing a PerkinElmer LC 300 liquid chromatography system with SimplicityChrom™ CDS and PerkinElmer Epic™ C18 SD column, the analysis is efficient and accurate, with tailing factor and repeatability results achieved are well within the monograph acceptance criteria.
Pregabalin is an anti-epileptic drug that works by slowing down impulses in the brain that cause seizures. It can also be utilized to treat fibromyalgia, or nerve pain in people with diabetes, herpes zoster, or spinal cord injury owing to its ability to affect chemicals in the brain that send pain signals across the nervous system. This application brief describes the analysis of Pregabalin, using the newly released LC 300 liquid chromatography system with SimplicityChrom™ chromatography data system (CDS) and PerkinElmer Epic™ C18 column in accordance with the USP monograph.
Paeoniae radix alba, also known by its Chinese phonetic name as Baishao, is a Traditional Chinese Medicine herbal plant used to treat inflammatory disorders. Paeoniflorin, the major active component extracted from Paeoniae radix alba, offers proven efficacy against gynecological disorders, pain, and hyperactivity of liver-yang, among other disorders.
To aid manufacturers in their quality control programs and protect consumers, Chinese Pharmacopoeia (2020 Edition) has finalized a method with the specification that paeoniflorin is not less than 1.6% in the herbal plant on a dry basis. In this work, quantification of paeoniflorin in herbal plants is performed using a PerkinElmer LC 300 HPLC/UHPLC system, Epic™ polar column, and SimplicityChrom™ software.
Feed milling today is a complex business, and agribusinesses both large and small need to balance the nutritional and safety needs of livestock with availability of raw ingredients and their seasonality and variability. Add to that the valuable supplementation and medication that millers supply, and it’s clear: you’re creating a complete nutritional delivery system, not just a simple mix of grains.
Laboratories in nearly every industry are continuously faced with growing demands for increased throughput, improved margins, and more efficient processes. And when it comes to liquid chromatography, a one-size-fits-all approach just isn’t enough. You need an LC system tailored to your needs, with user-friendly software, consumables, service, and support – the total package.
Meet the PerkinElmer LC 300, the latest LC to step off our production lines. With features and benefits such as five available detectors, ultraprecise gradient flows, low dispersion, and new Simplicity™ Chrom CDS software, our LC 300 systems provide improved workflows, throughput, and usability.
The Epic™ line is our latest range of LC columns, based on high density monomerically bonded phases produced through a proprietary bonding process. Epic HPLC and UHPLC columns are compatible with a wide range of organic modifiers and buffers and stable over a wide pH range. All Epic products use an ultra-high purity metal free silica and undergo strict quality control testing. For flexibility, we offer a wide range of column sizes as well being fully scalable from analytical to preparative dimensions. Whatever your separation need, we have a chemistry or dimension to fill it.
SimplicityChrom™ is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and support, and you’re free to focus on what matters most – your analytical goals.
The PerkinElmer LC 300 liquid chromatography systems are the result of listening to user needs, and paying careful attention to every detail in the resulting design. The LC 300 systems are the ideal solution for labs faced with high throughput requirements or challenging chromatographic separations.
Whatever the application, the PerkinElmer LC 300 system’s performance and enhanced user experience will ensure your lab’s success, whether that need be for pharmaceutical, food, consumer products or specialty chemicals.
In this product note, detailed information on each of the LC 300's modules is presented.
The most commonly used LC reversed-phase alkyl bonded stationary phase is octadecyl carbon chain (C18)-bonded silica, which is denoted as USP classification L1. C18 columns have a broad applicability from pharmaceuticals to food and environmental analyses. However, not all C18 columns are alike. Simply swapping a C18 column from one manufacturer to another can result in differences in retention time, resolution and even selectivity. Differences can arise due to variations in hydrophobicity, silanol activity, packing quality, particle size distribution, and silica purity, to name a few.
This technical note provides details of a comparative study between twenty-four silica-based C18 phases, from a number of manufacturers covering the following areas:
Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with compliant procedures, any unplanned downtime to investigate OOS instances can impact productivity. Our range of clone phases offer a cost-effective comparable alternative to many of the older leading brands, whilst ensuring consistency and stability in analysis. Better lot-to-lot reproducibility is also achieved due to more stable production methods especially when compared to older brands. Our Aviator™ line exhibits similar selectivity to Avantor ACE® HPLC columns. Aviator C18 provides a rugged, reproducible starting point for method development and applications with analytes differing in hydrophobicity, polar, moderately polar and non-polar analytes, uncharged acids and bases, and ionized acids or bases using ion-pairing. Other Aviator phases are also available including AQ, C8, C4, Cyano and Phenyl. This technical note provides examples of comparative studies between the Aviator C18 (150 x 4.6 mm, 5 µm) column and the ACE C18 (150 x 4.6 mm, 5 µm) column, for the following pharmaceutical drug applications:
Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with compliant procedures, any unplanned downtime to investigate OOS instances can impact productivity. Our range of clone phases offer a cost-effective comparable alternative to many of the older leading brands, whilst ensuring consistency and stability in analysis. Better lot-to-lot reproducibility is also achieved due to more stable production methods especially when compared to older brands. Our Sonoma™ line is an alternative to Phenomenex Luna®. Excellent efficiencies, peak shape and resolution are obtained for virtually all Luna HPLC applications from high quality Sonoma HPLC columns. A range of particle sizes offers versatility from analytical and LC/MS to prep and process scale applications. Sonoma C18(2), the most popular phase, is equivalent to Luna C18(2). Available in 3 µm and 5 µm particle sizes, with bulk and preparative material available. Other Sonoma phases available include C18, C5, C8, C8(2), Cyano, HILIC, NH2 (amino), PFP(2), Phenyl-Hexyl and Silica(2). This technical note displays examples of comparative studies between our Sonoma C18(2) (150 x 4.6 mm, 5 µm) column and the Phenomenex Luna C18(2) (150 x 4.6 mm, 5 µm) column, for the following pharmaceutical drug applications:
Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with compliant procedures, any unplanned downtime to investigate OOS instances can impact productivity. Our range of clone phases offer a cost-effective comparable alternative to many of the older leading brands, whilst ensuring consistency and stability in analysis. Improved lot-to-lot reproducibility is also achieved due to more stable production methods especially when compared to older brands.
The HarmonyTM C18 provides equivalent selectivity to the Waters Symmetry® C18 HPLC column and many chromatographers use Harmony when looking for a Symmetry equivalent. Harmony offers excellent column lifetime and reproducibility, and is engineered to the tightest specifications. It is also available in large scale bulk. Other Harmony phases are also available including C8 and C4.
This technical note provides examples of comparative studies between our Harmony C18 and the Waters Symmetry C18 phases for the following pharmaceutical drug applications:
The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed radial groove in the rotor seal which ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.