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The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
TSH (thyroid stimulating hormone), also known as thyrotropin, is a member of the cysteine knot growth factor superfamily. It is a heterodimer consisting of a 15 kDa unique TSH beta subunit and a 14 kDa alpha subunit, common glycoprotein hormone alpha that is shared with Luteinizing Hormone, Follicle Stimulating Hormone and Human Chorionic Gonadotropin. Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH. TSH binds to thyroid TSH receptors to stimulate production of thyroxine (T4). In tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which stimulates the metabolism of almost every tissue in the body and completes a feedback loop by inhibiting TSH production. Serum TSH measurement is a crucial tool for the diagnosis of thyroid disorders. Increased serum TSH is an early and sensitive indicator of decreased thyroid reserve and overt primary hypothyroidism. Decreased TSH level is an indicator of TSH-independent hyperthyroidism (Graves' disease). For recombinant hTSH, conversion for IU is 1 µg of protein equals 7.9 x 10-3 IU.
|Assay Target Class||Hormone|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.