LANCE Ultra total STAT1 kits are designed for the detection of STAT1 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-STAT1 assay.
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Please note control lysates are sold separately, catalog number TRF4028S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
STAT1 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT1 is phosphorylated at Tyr-701 by receptor associated kinases, upon which STAT1 will dimerize (sometimes forming a heterodimer with STAT3), translocate to the nucleus, and work as transcription factors. Mutations of STAT1 result in either gain of function (resulting in CMC) or loss of function (causing impaired response to infection).
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual describes how to run the LANCE Ultra cellular total STAT1 assay kit.