LANCE Ultra phospho-Src (Tyr419) kits are designed for the detection of Src (phosphorylated at residue Y419) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF4018C||500 Assay Points||1450.00 USD|
|TRF4018M||10,000 Assay Points||6500.00 USD|
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Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4018S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
Src is a protein tyrosine kinase which is involved in regulating differentiation as well as cell growth. Unusual tumor growth is observed in cancers where Src is activated due to the promotion of cell survival, angiogenesis, proliferation and invasion pathway. Src can be phosphorylated at multiple sites, Phosphorylation at Y419 has been shown to upregulate Src kinase activity.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Phosphoprotein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 77 KB|
|Guide||LANCE Ultra TR-FRET Cellular Phosphorylation Quick Start Guide||PDF 1 MB|
|Application Note||LANCE Ultra TR-FRET-based Detection and Modulation of Phosphorylated STAT3 Levels in Human Cells||PDF 2 MB|
|Manual||Protocol for LANCE Ultra TR-FRET phospho-Src cellular detection assay||PDF 482 KB|
|Technical Note||Development of LANCE TR-FRET Kits for Cellular Phosphoprotein Detection||PDF 1 MB|
|Technical Note||Optimization of LANCE Ultra Cell-based Phosphorylated ERK Assay Using the EnSight||PDF 1 MB|