LANCE Ultra phospho-SMAD1 (Ser463/465) kits are designed for the detection of SMAD1 (phosphorylated at S463/465) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF4011C||500 Assay Points||1450.00 USD|
|TRF4011M||10,000 Assay Points||6500.00 USD|
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Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4011S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
SMAD proteins are transcriptional activators activated by bone morphogenic proteins (BMPs). These ligands induce dimerization of SMAD proteins, whereby they translocate to the nucleus and bind target genes. SMAD1, 2, and 3 constitute receptor-regulated SMADs.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Phosphoprotein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 82 KB|
|Guide||LANCE Ultra TR-FRET Cellular Phosphorylation Quick Start Guide||PDF 1 MB|
|Application Note||LANCE Ultra TR-FRET-based Detection and Modulation of Phosphorylated STAT3 Levels in Human Cells||PDF 2 MB|
|Manual||Protocol for LANCE Ultra TR-FRET phospho-SMAD1 cellular detection assay||PDF 462 KB|
|Technical Note||Development of LANCE TR-FRET Kits for Cellular Phosphoprotein Detection||PDF 1 MB|