LANCE Ultra phospho-TBK1 (Ser172) kits are designed for the detection of phosphorylated TBK1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
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For research use only. Not for use in diagnostic procedures.
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Please note control lysates are sold separately, catalog number TRF4031S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
TANK-binding kinase 1 (TBK1) is auto-phosphorylated at Ser172. It contributes to multiple pathways. Activation of TBK1 leads to the activation of the TLR3/4 and cGAS-STNG signaling pathways. Ultimately activation of TBK1 leads to IRF3 activation. Phosphorylation of optineurin and p62 by TBK1 marks proteins to be directed to the autophagosome.
|Assay Target Class||Phosphoprotein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
Manual and sample data for LANCE Ultra phospho-TBK1 detection kit.