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Phospho-TBK1 (Ser172) LANCE Ultra TR-FRET Detection Kit, 500 assay points

LANCE Ultra phospho-TBK1 (Ser172) kits are designed for the detection of phosphorylated TBK1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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500 Assay Points
1557.00 USD
10,000 Assay Points
7000.00 USD
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Detail Information

Please note control lysates are sold separately, catalog number TRF4031S.


  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

TANK-binding kinase 1 (TBK1) is auto-phosphorylated at Ser172. It contributes to multiple pathways. Activation of TBK1 leads to the activation of the TLR3/4 and cGAS-STNG signaling pathways. Ultimately activation of TBK1 leads to IRF3 activation. Phosphorylation of optineurin and p62 by TBK1 marks proteins to be directed to the autophagosome.


Assay Target TBK1
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 500 Assay Points