LANCE Ultra phospho-TBK1 (Ser172) kits are designed for the detection of phosphorylated TBK1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Item(s) added to cart.
For research use only. Not for use in diagnostic procedures.
Please enter valid quantity
Please log in to add favorites.
NULL OR EMPTY CART
Please note control lysates are sold separately, catalog number TRF4031S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
TANK-binding kinase 1 (TBK1) is auto-phosphorylated at Ser172. It contributes to multiple pathways. Activation of TBK1 leads to the activation of the TLR3/4 and cGAS-STNG signaling pathways. Ultimately activation of TBK1 leads to IRF3 activation. Phosphorylation of optineurin and p62 by TBK1 marks proteins to be directed to the autophagosome.
|Assay Target Class||Phosphoprotein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
Manual and sample data for LANCE Ultra phospho-TBK1 detection kit.