PER.C6 HCP LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra PER.C6 HCP Detection Kit is designed for detection and quantitation of PER.C6 host cell protein contaminants in bioprocess samples using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

The PER.C6® human cell line is utilized as an expression system for the industrial scale production of human recombinant proteins or biotherapeutics. The PER.C6® cell line has several advantages over other platforms, such as increased yield and improved safety of the final product. Biotherapeutics for human use must be extensively purified to remove undesired contaminants, such as host cell proteins, that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.