The LANCE® Ultra Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF1521C||500 Assay Points||796.00 USD|
|TRF1521M||10,000 Assay Points||10600.00 USD|
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The LANCE Ultra Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
In the mouse, leptin is a hormone that contains 146 amino acid residues and is secreted by differentiated adipocytes. It regulates energy homeostasis as a result of its action on the brain via hypothalamic neuronal pathways expressing the leptin receptor (LR). Hereditary deficiency of leptin, or functional LRs, causes severe obesity in humans and mice. Leptin mediated signaling has been implicated in the regulation of food intake, energy expenditure, lipid and carbohydrate metabolism, and reproductive, neuroendocrine, thyroid, and immune function.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Hormone|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 77 KB|
|Application Note||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|
|Technical Note||Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines||PDF 277 KB|
|Data Sheet||LANCE Ultra Mouse Leptin Detection Kit TRF1521||PDF 458 KB|
|Flyer||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|