PerkinElmer
check quantity

IL-1RA (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

You successfully added item(s) to your cart

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
TRF1358C
500 Assay Points
892.00 USD
more
TRF1358M
10,000 Assay Points
11900.00 USD
more
Buy Now

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Detail Information

The LANCE® Ultra Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interleukin-1 Receptor Antagonist (IL1RA) is a member of the interleukin-1 cytokine family. Immune, epithelial, and adipose cells secrete IL1RA which will bind to the interleukin-1 receptor and inhibit pro-inflammatory signaling through IL-1α and IL-1β. Studies have shown that IL1RA plays a key role in suppressing the pro-inflammatory effects of IL-1 in diseases like rheumatoid arthritis. Additionally, it has been observed that IL1RA may play an important role in diseases like osteoporosis where IL1RA levels are suppressed compared to controls. Furthermore, it has been observed that IL-1 cytokines are abundantly present at tumor sites and the addition of IL1RA can suppress growth of IL-1 producing tumors.

Specifications

Assay Target IL-1RA
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 10,000 Assay Points
Resources, Events & More
  • All

Brochure

Manual

LANCE Ultra Human IL-1RA Detection Kit TRF1358

This manual explains how to run the LANCE Ultra human IL-1RA detection assay.

PDF 480 KB