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IL-31 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human IL-31 Detection Kit is designed for detection and quantitation of human interleukin-31 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF3003C
500 Assay Points
773.00 USD
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TRF3003M
10,000 Assay Points
10300.00 USD
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Detail Information

The LANCE® Ultra Human IL-31 Detection Kit is designed for detection and quantitation of human IL-31 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interleukin 31 (IL31), a cytokine with a four-helix bundle structure, is produced by type 2 helper T cells and belongs to the IL6 cytokine family. Recently, several studies reported on prominent functions of the novel T-cell-derived pro-inflammatory cytokine IL31 and the levels in serum associated with patient having asthma, chronic inflammation, and skin diseases. Assaying for levels of IL31 can be used to measure and monitor the amount of inflammation and to guide inflammatory disease treatment.

Specifications

Assay Target IL-31
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 500 Assay Points
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Brochure

Manual

LANCE Ultra Human IL-31 Detection kit TRF3003

This manual explains how to run the LANCE Ultra TR-FRET human MMP-7 detection assay.

PDF 495 KB