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The LANCE® Ultra Human IgM Detection Kit is designed for detection and quantitation of human IgM in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Immunoglobulin M (IgM) is the largest antibody produced by B cells. It is mainly produced in a pentameric form weighing around 900 kDa, but also in a hexameric form. Its multimeric nature gives IgM high avidity. It is mostly found in the serum, yet it is also present in secretions. IgM is a very efficient activator of the complement and appears very early during the infection process. Moreover, anti-A and anti-B antibodies responsible for blood type incompatibility are IgM molecules. Selective IgM deficiency (SIgMD) is a very rare immune disorder that results in absence or deficiency of IgM, with normal levels of other immunoglobulins, namely IgA and IgG. Hyper-IgM syndrome (HIGM) is characterized by a decrease or absence of IgG and IgA, whereas the IgM level is normal or increased.
|Assay Target Class||Antibody|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Dry Ice|
|Unit Size||10,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.