The LANCE® Ultra PIP Collagen Detection Kit is designed for detection and quantitation of human PIP collagen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF1353C||500 Assay Points||773.00 USD|
|TRF1353M||10,000 Assay Points||10300.00 USD|
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The LANCE® Ultra PIP Collagen Detection Kit is designed for detection and quantitation of PIP collagen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Procollagen Type I C-peptide (PIP), also known as Procollagen I Carboxy Terminal Propeptide (PICP), is an enzymatically cleaved product of pro-collagen type I. Cleavage of PIP is required for the initiation of fibril formation. PIP has been used as an indicator of the synthesis of type I collagen. PIP is a trimeric, globular protein consisting of three polypeptide chains, two proα1 (I) and one proα2 (I) chains with molecular mass of PIP of 100 KD. The levels of PIP in biological samples is directly associated with numerous pathological states including bone, liver, heart diseases.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target||PIP Collagen|
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 82 KB|
|Application Note||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|
|Manual||LANCE Ultra PIP Collagen Detection kit TRF1353||PDF 542 KB|
|Poster||Comparison of EMT biomarker expression in 2D monolayer and 3D spheroid cultures in a prostate cancer||PDF 1 MB|
|Flyer||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|