PIP Collagen (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra PIP Collagen Detection Kit is designed for detection and quantitation of PIP collagen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Procollagen Type I C-peptide (PIP), also known as Procollagen I Carboxy Terminal Propeptide (PICP), is an enzymatically cleaved product of pro-collagen type I. Cleavage of PIP is required for the initiation of fibril formation. PIP has been used as an indicator of the synthesis of type I collagen. PIP is a trimeric, globular protein consisting of three polypeptide chains, two proα1 (I) and one proα2 (I) chains with molecular mass of PIP of 100 KD. The levels of PIP in biological samples is directly associated with numerous pathological states including bone, liver, heart diseases.