The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
true falseItem(s) added to cart.
For research use only. Not for use in diagnostic procedures.
The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Insulin is synthesized as a proinsulin hormone of 110 aa by Beta-cells of the islets of Langherans in the pancreas. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A of 21 aa and peptide B of 30 aa) that are covalently linked via two disulfide bonds and secreted upon increased glucose concentration in blood. Blood concentration increases from around 50 pmol/L to 300-400 pmol/L 30 min after glucose uptake. Insulin is a key player in the control of both carbohydrate and lipid metabolism and has been implicated in various diseases including diabetes, heart disease and obesity.
Assay Target | Insulin |
---|---|
Assay Target Class | Hormone |
Automation Compatible | Yes |
Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
Experimental Type | In vitro |
Shipping Condition | Blue Ice |
Target Species | Human |
Therapeutic Area | Metabolic |
Unit Size | 10,000 Assay Points |