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The LANCE® Ultra Human FSH Detection Kit is designed for detection and quantitation of human follicle stimulating hormone (FSH) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Follicle Stimulating Hormone (FSH) is a 35.5 kDa heterodimer (alpha and beta unit) glycoprotein that is synthesized by the anterior pituitary gland and regulates the fertility processes. The FSH molecule shares a common subunit (alpha chain) with Luteinizing Hormone (LH), Thyroid-Stimulating Hormone (TSH), and Human Chorionic Gonadotropin (hCG) while the beta subunit of FSH varies from LH, TSH, and hCH. The beta subunit is known to carry out specific biological activities on the ovary and testis. The level of FSH in serum is an excellent indicator for determining the status of oocyte maturation and follicle development in female. Infertility in females is often associated with mutations of the FSH gene resulting in reduced FSH synthesis. FSH is measured in International Units (IU). For recombinant FSH, one IU corresponds to approximately 0.065 to 0.075 µg of protein.
|Assay Target Class||Hormone|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
This manual explains how to run a LANCE Ultra TR-FRET detection assay for human follicle stimulating