LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF4000C||500 Assay Points||1450.00 USD|
|TRF4000M||10,000 Assay Points||6500.00 USD|
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Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4000S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
ERK1 and ERK2, also known as MAP Kinase 3 and MAP Kinase 1, are serine/threonine kinases and important targets for cancer treatment. ERK kinases are dually phosphorylated at Tyr-202 and Tyr-204, leading to activation of the target. ERK can also be dephosphorylated at Tyr-202 by PTPRJ. A number of downstream targets have been identified including EIF4EBP1, which is necessary for the initiation of translation. It has also been shown to phosphorylate ELK-1.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Phosphoprotein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 82 KB|
|Guide||LANCE Ultra TR-FRET Cellular Phosphorylation Quick Start Guide||PDF 1 MB|
|Application Note||LANCE Ultra TR-FRET-based Detection and Modulation of Phosphorylated STAT3 Levels in Human Cells||PDF 2 MB|
|Technical Note||Development of LANCE TR-FRET Kits for Cellular Phosphoprotein Detection||PDF 1 MB|
|Technical Note||Optimization of LANCE Ultra Cell-based Phosphorylated ERK Assay Using the EnSight||PDF 1 MB|