PerkinElmer
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EGF (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human EGF Detection Kit is designed for detection and quantitation of human epidermal growth factor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1339C
500 Assay Points
796.00 USD
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TRF1339M
10,000 Assay Points
10600.00 USD
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Detail Information

The LANCE® Ultra Human EGF Detection Kit is designed for detection and quantitation of human epidermal growth factor (EGF) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Epidermal Growth Factor (EGF) is a 6.2 kDa protein ligand that binds with high affinity to the epidermal growth factor receptor. Initially EGF is expressed as pro-EGF and is later cleaved to its mature form by enzymes and released via exocytosis. EGF, through EGFR, has been observed to play a role in initiating cell growth, proliferation and differentiation. Dysregulation of EGF levels has been linked to several diseases including but not limited to ovarian cancer and diabetes.

Specifications

Assay Target EGF
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Unit Size 10,000 Assay Points