PerkinElmer
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Cortisol LANCE Ultra TR-FRET Detection Kit, 500 assay points

The LANCE® Ultra Cortisol Detection Kit is designed for detection and quantitation of cortisol in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1314C 500 Assay Points 1512.00 USD
TRF1314M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Cortisol Detection Kit is designed for detection and quantitation of cortisol in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with an acceptor fluorophore [ULight dye]. A "tracer" analyte is biotinylated and binds Streptavidin labeled with donor Eu-chelate. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. In the presence of sufficient non-biotinylated analyte this emission will be diminished.

Cortisol (hydrocortisone) is a steroid derived from cholesterol. Cortisol can be found in the blood as free or bound to cortisol-binding globulins, such as transcortin. Cortisol levels follow a diurnal pattern, typically highest in the morning upon wake up and lowest just before sleep. Cortisol levels have been linked to metabolism regulation, glucogenesis promotion, liver glycogen deposition, and depression of glucose burn. Cortisol levels have also been positively correlated to stress and increasing blood pressure.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Cortisol
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual Manual: LANCE Ultra Cortisol Detection Kit PDF  559 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB