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ApoE (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human ApoE Detection Kit is designed for detection and quantitation of human Apolipoprotein E (ApoE) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1395C 500 Assay Points 750.00 USD
TRF1395M 10,000 Assay Points 10000.00 USD
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Overview

The LANCE® Ultra Human ApoE Detection Kit is designed for detection and quantitation of human apolipoprotein E (ApoE) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Apolipoprotein E (ApoE) is produced in the liver, brain, kidneys, and spleen. ApoE is mostly found in the intermediatedensity lipoproteins. A critical component of triglyceride-rich lipoproteins, it transports lipoproteins, fat-soluble vitamins, and cholesterol into the tissues and then into the blood. This protein is initially recognized for its importance in lipoprotein metabolism and cardiovascular disease. Recently, ApoE has been shown to be involved in Alzheimer's disease, immunoregulation, and cognition.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target ApoE
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human ApoE Detection Kit TRF1395 PDF  509 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB