For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein (ANGPTL4) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Angiopoietin-like 4 (ANGPTL4), also known as HFARP, PGAR, or FIAF, is a 55 kDa glycoprotein that is structurally related to the angiopoietin family. It is secreted by the liver and fat tissue, and contains an N-terminal coiled-coil domain, and taC-terminal fibrinogen-like domain. Angiopoietin-like 4 plays a role in lipoprotein metabolism and angiogenesis. It is also involved in numerous cancers and is implicated in the metastatic process by modulating vascular permeability, cancer cell motility and invasiveness.
| Assay Target | ANGPTL4 |
|---|---|
| Assay Target Class | Protein |
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Angiogenesis |
| Unit Size | 10,000 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
This manual explains how to run the LANCE Ultra TR-FRET human ANGPTL4 detection assay.
OptiPlate-384, White Opaque 384-well Microplate, Case 50
List Price : USD295.0
List Price : USD67.0
