PerkinElmer
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LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100), 1 mg

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.

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AD0203
10 µg
215.00 USD
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AD0161
50 µg
1043.00 USD
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AD0162
1 mg
9700.00 USD
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Detail Information

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-P-Tyr-100
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 1 mg
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Application Note

Measurement of p38/MAPK Activity Using LANCE

Protein kinases regulate several important functions within cells including metabolism, cell cycle progression, angiogenesis, cell adhesion, etc. Specifically, mitogen-activated protein kinases (MAPK) play a central role in the cellular response to environmental stress, growth factors, and cytokines.

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Brochure

Manual

LANCE generic reagents

Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.

PDF 101 KB

Poster

EGFR and human EphA4 receptor tyrosine kinaseassays for compound screening: Performance evaluation of LANCE®Ultra?TR-FRET assay platform

LANCE Ultra and Supplier I TR-FRET reagents were compared for their capacity to deliver robust performance for EGFR and EphA4 kinase screening.

PDF 159 KB