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LANCE Eu-W1024 Anti-c-Myc, 10 µg

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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Unit Size
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AD0206
10 µg
324.00 USD
 
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AD0114
50 µg
1275.00 USD
 
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AD0115
1 mg
15600.00 USD
 
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Overview

LANCE® Eu-labeled anti-c-myc antibodies are purified mouse IgG1 monoclonal antibodies, which recognize the epitope sequence (EQKLISEEDL) derived from the human c-myc protein. These monoclonal antibodies against the c-myc epitope do not cross react with other cellular proteins.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-c-Myc
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Product Brand Name LANCE
Shipping Condition Dry Ice
Unit Size 10 µg
Resources, Events & More
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.

PDF 1 MB

Brochure

Manual

LANCE generic reagents

Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.

PDF 101 KB