PerkinElmer
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LANCE Eu-W1024 Anti-6xHis, 1 mg

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

This is a replacement for AD0111.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AD0400
1 mg
12100.00 USD
 
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AD0401
50 μg
1214.00 USD
 
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AD0402
10 μg
256.00 USD
 
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Overview

Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.

LANCE (Lanthanide chelate excite) and LANCE® Ultra™ are TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (˜10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-6X His
Assay Technology Time-Resolved Fluorescence (TRF), TR-FRET
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Product Brand Name LANCE
Shipping Condition Dry Ice
Unit Size 1 mg