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IVIS SpectrumCT In Vivo Imaging System

The IVIS® SpectumCT preclinical in vivo imaging system expands upon the versatility of the IVIS Spectrum by offering 2D and 3D imaging capabilities but includes integrated low-dose microCT ideal for longitudinal studies. The system provides researchers with greater insights into complex biological systems by enabling simultaneous molecular and anatomical non-invasive imaging in animal models.

Part Number 128201
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For research use only. Not for use in diagnostic procedures.

Detail Information

The constant horizontal gantry motion and the flat panel detector provide unparalleled performance for low-dose imaging and automated optical and microCT integration. The stable revolving animal platform table rotates 360° to acquire full 3D data. Multiple animals can be scanned simultaneously while maintaining an average dose per scan at about 13mGy, with a scanning and reconstruction time of less than a minute. Optical and microCT modalities can also operate independently.

Key features include:

  • Integrated optical and microCT technology
  • 3D optical tomography for fluorescence and bioluminescence
  • The industry’s most sensitive detection technology ideal for:
    • Bioluminescence
    • Multispectral fluorescence and spectral unmixing
    • Cerenkov imaging for optical radiotracer imaging
    • Low dose and ultra fast microCT
    • DyCE dynamic enhanced imaging for real time distribution studies of both fluorochromes or PET tracers ideal for PK/PD app

The IVIS SpectrumCT is an integrative platform that combines the full suite of IVIS optical features including Spectral Unmixing, 2D and 3D quantitative bioluminescence and fluorescence with fast and low dose CT imaging. The simple user interface along with automated co-registration, advanced visualization and analysis tools are driven by PerkinElmer’s market leading Living Image® software. The IVIS Spectrum CT enables longitudinal workflows to characterize disease progression and therapeutic effect throughout the complete experimental time frame with both quantitative CT and optical reconstructions. Fast imaging and the ability to image multiple animals offers the throughput required to scan large cohorts of animals quickly and draw sound conclusions from your experimental data.

Specifications

Imaging Modality Optical Imaging, microCT Imaging
Optical Imaging Classification Bioluminescence imaging, Fluorescence Imaging
Portable No
Product Brand Name IVIS
Resources, Events & More
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Application Note

Cerenkov Imaging of Radioisotopes in IVIS systems

Cerenkov Emission from radioisotopes in tissue,Optical imaging detects photons in the visible range of the electromagnetic,spectrum. PET and SPECT imaging instruments are sensitive to photons in the much,higher energy range of x-rays and gamma rays. While the PET and SPECT probes,which can generate Cerenkov light in tissue will continue to produce the relevant,gamma- and x-rays, visible photons will be produced from the Cerenkov emission,which the IVIS® will detect.,In beta decay emitters such as PET probes and isotopes such as 90Y, 177Lu, 131I and 32P,the beta particle will travel in the tissue until it either annihilates with an electron or,loses momentum due to viscous electromagnetic forces.,It is possible that the beta (electron or positron),is relativistic, traveling faster than the speed,of light in the tissue. While it is impossible,to travel than the speed of light in a vacuum,(c), the speed of light in tissue is v = c / n,where n is the tissue index of refraction and,n = 1. Cerenkov photons will be generated,by a relativistic charged particle in a dielectric medium such as tissue.

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Imaging Hepatocellular Liver Injury using NIR-labeled Annexin V

Drug induced liver injury (DILI) is a major reason,for late stage termination of drug discovery,research projects, highlighting the importance,of early integration of liver safety assessment in the drug development process. A technical,approach for in vivo toxicology determination was developed using Acetaminophen (APAP),a commonly used over-the-counter analgesic and antipyretic drug, to induce acute,hepatocellular liver injury. PerkinElmer imaging technology and near infrared (NIR) labeled,Annexin V (Annexin-VivoTM 750) were used to detect and quantify and necrosis associated,with this type of liver toxicity. Both fluorescent tomographic imaging (FMT® 4000 and IVIS®,SpectrumCT), and higher throughput epifluorescence imaging (IVIS SpectrumCT), provided,excellent detection of Annexin-Vivo fluorescence in the liver. Histology and plasma alanine,transaminase (ALT) confirmed the kinetics of tissue necrosis, and more extensive liver damage,was seen but with an apparent decrease in tissue PS and plasma ALT by 48 hours, suggesting,a decline in the induction of tissue destruction. Compared to conventional plasma/serum,assays, in vivo imaging can offer fast, quantitative imaging results directly assessing the tissue,of interest.

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Multiplex 2D Imaging of NIR Molecular Imaging Agents on the IVIS SpectrumCT and FMT 4000

Epifluorescence (2D) imaging of superficially,implanted mouse tumor xenograft models offers,a fast and simple method for assessing tumor,progression or response to therapy. This approach,for tumor assessment requires the use of near,infrared (NIR) imaging agents specific for different,aspects of tumor biology, and this Application,Note highlights the ease and utility of multiplex,NIR fluorescence imaging to characterize the,complex biology within tumors growing in a living,mouse. IntegriSense™ 750 detects avb3 integrin,expression, BombesinRSense™ 680 detects the upregulation of bombesin receptor,(associated with tumor proliferation), Transferrin-Vivo™ 750 detects increases in,transferrin receptor (due to increased iron metabolism), MMPSense® 680 is activated,by disease-related matrix-metalloproteases (secreted by tumor cells and tumor,associated macrophages), and ProSense® 750EX detects increases in cathepsin,activity (elevated in lysosomes of tumors and inflammatory cells). OsteoSense® 680,a bone turnover imaging agent, was used as a non-tumor imaging control. These,and multiple other PerkinElmer imaging agents, can be used to characterize tumor,biology, and in this set of studies the data shows that two different tumors, HeLa,(cervical cancer) and 4T1 (breast cancer), can differ in their pattern of labeling,intensities for six distinct biological imaging agents. Such an approach is likely to,prove valuable for the full biological characterization of tumors during progression,metastasis, or response to treatment.

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Brochure

3D Multimodality Imaging: See disease in all its dimensions

It’s simple: More information means more understanding,For today’s researchers in oncology, infectious diseases, inflammation, neuroscience, stem cells,and other disciplines, there’s an increasing need for in vivo imaging that enables you to visualize,multiple events simultaneously and to extract the maximum amount of information from each,subject – leading to greater biological understanding.,Multimodal imaging enables a better understanding of disease biology. By utilizing in vivo,optimized bioluminescent and fluorescent agents and radioactive probes, researchers can,measure depth, volume, concentration, and metabolic activity, providing a wealth of information,for untangling the mysteries of disease.,Coregistration allows researchers to overlay images from multiple imaging modalities, providing,more comprehensive insight into the molecular and anatomical features of a model subject.,For example, optical imaging data can be used to identify and quantify tumor burden at,the molecular level and, when integrated with microCT, provides a quantitative 3D view of,anatomical and functional readouts.,At PerkinElmer, we’ve developed industry leading imaging technology for preclinical research.,Our technology integrates 3D optical and PET modalities with microCT to provide a better,understanding of disease. And that means better monitoring of disease progression, earlier,detection of treatment efficacy, and deeper understanding of metabolic changes that take place,throughout disease development.

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Event

Poster

A fluorescent agent cocktail for detecting both cholestasis and hepatocellular forms of acute drug-induced liver injury

Drug induced liver injury (DILI) is a major reason for late stage,termination of drug discovery research projects, so assessment is,being integrated earlier in the drug development process. Some,chemicals can produce different forms of hepatic injury in mice,including the two most common forms, cholestasis and,hepatocellular injury. Biochemical serum markers of liver damage,like alanine transferase (ALT) and alkaline phosphatase (ALP) are,limited in their ability to detect both of these common forms of liver,injury and focus on plasma as an indirect measure of what is,occurring in the liver.

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Highly Sensitive Lanthanide Based Luminescent Particles for In Vivo Imaging of ROS Species in Deep Tissues

Introduction: Reactive oxygen species (ROS) play a critical role in a wide variety of disease conditions like cancer, inflammation, neurodegenerative disorders and oxidative stress. Highly sensitive and specific optical probes (fluorescent, luminescent or chemiluminescent probes) are therefore required for detecting and studying the roles of different ROS in disease pathogenesis. However, very short life times of these species coupled with the presence of antioxidants in living systems make it extremely hard to detect these reactive species in vivo, especially in deep tissues. We employed the chemiluminescent properties of lanthanide acceptor beads to develop a highly sensitive probe for ROS detection by non-invasive optical imaging. In this approach when an acceptor bead comes in close proximity (200nm) to Singlet oxygen (1O2), energy is transferred from the singlet oxygen to thioxene derivatives within the acceptor bead, resulting in light production at 520-620 nm (EPRM®). The major advantages of this approach are: a. enabling detection of ROS by generating long-lived signal (half-life in seconds); b. Achieving high sensitivity due to lack of background signal and c. Generating long wavelength (620nm) signal thereby allowing deep tissue interrogations in living organisms.

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In vivo fluorescent imaging of tumor bombesin and transferrin receptor expression as early indicators of sorafenib efficacy in small animal models

Targeted cancer therapy aims to block key signaling pathways that are critical for tumor cell growth and survival. The blockage eventually results in cell death via apoptosis and eventual tumor growth suppression. This strategy has proven to be quite effective, and the FDA has approved several targeted therapeutics in the past decade. Encouraged by the success in clinical development, many academic and pharmaceutical researchers are in active pursuit of improved next generation targeted anti-cancer drugs. As a result, many new chemical and biological entities are emerging from initial screening of in vitro, in vitro and/or in silico selection processes. From the perspective of drug development, it poses a great challenge for the next stage of in vivo validation and demands a robust, accurate, and efficient method for assessment of these candidates in living animal models.

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Iterative Reconstruction Approach to Minimize Metal Artifacts in a Rotating Turntable CT System

Visualization and quantification of Computed Tomography (CT) scans is ideally performed on artifact free images. Materials with a high linear attenuation coefficient, such as metal, cause significant artifacts in the reconstructed image. Unfortunately, the use of metal is unavoidable in some orthopaedic and dental models and with some animal tracking systems.,Many iterative reconstruction approaches used in the past remove metal from the sinogram before the final reconstruction. These sinograms are geometry dependent, but the algorithms have not been tested for the rotating turntable geometries used in some preclinical uCT systems. These preclinical uCT systems also have specific image processing needs to facilitate specific co-registration applications.

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Molecular imaging of tumor energy metabolism as an early indicator of anti-cancer drug efficacy in small animal models

Targeted cancer therapy aims to block key signaling pathways that are critical for tumor cell growth and survival. The blockage eventually results in cell death via apoptosis and tumor growth suppression. This strategy has proven to be safe and efficacious, and the FDA has approved several targeted therapeutics in the past decade. Encouraged by the success in clinical development, many academic and pharmaceutical researcher are in active pursuit of the improvement of next generation targeted anti-cancer drugs. As a result, many new chemical and biological entities are emerging from initial screening of in vitro, in vitro and/or in silico selection processes. From the perspective of drug development, it poses a great challenge on the next stage of in vivo validation and demands a robust, accurate, and efficient method for assessment of these candidates in living animal models.,Cancer cells are known to have abnormally increased cellular metabolism, and in the early stages of effective drug treatment, cancer cells show decreases in metabolism and proliferation. These events occur prior to overt signs of cell destruction and bioluminescence imaging technology can be used to detect early changes in tumor viability in response to targeted therapy when using luciferase-expressing tumor cell lines. However, with many therapeutic treatments, particularly high-dose treatments, these early changes in tumor viability cannot be seen by bioluminescence imaging. We applied non-invasive near infrared (NIR) fluorescence imaging to address this challenge, using PerkinElmer’s imaging agents; highly metabolic cancer cells have accompanying elevations in receptors for bombesin and transferrin on their surface that can be readily imaged using our fluorescent agents, BombesinRSenseTM 680 [BR680] and Transferrin-VivoTM 750 [TV750], respectively. In this report, we demonstrate the synergistic use of fluorescence (FLI) and bioluminescence imaging (BLI) to profile tumor metabolism and vitality, respectively, in response to a targeted anti-cancer drug, sorafenib. Sorafenib is a clinically approved tyrosine kinase inhibitor effectively blocking VEGFR, PDGFR and Raf signaling in cancer. At a higher daily dose of 120 mg/kg, the drug effectively decreases viability of HCT116-luc human colon xenograft tumors within 48 hours, coinciding with the loss of bioluminescence as well as BR680 and TV750 fluorescent signals in tumors. Interestingly, at a lower dose of 40 mg/kg, BR680 signal reduction can be observed early (within 48 hours), but no significant reduction of tumor viability by BLI and tumor volume was observed until a week later. These results suggest the potential use of metabolic fluorescent imaging agents as robust, efficient and early biomarkers for pre-clinical development of targeted cancer drugs.

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Product Note

IVIS SpectrumCT Product Note

The IVIS® SpectrumCT expands upon the versatility,and advanced optical feature sets of the IVIS and,Maestro™ platforms integrated with low dose,microCT to support longitudinal imaging. The IVIS,SpectrumCT enables simultaneous molecular and,anatomical longitudinal studies, providing researchers,with essential insights into complex biological,systems in small animal models. The constant horizontal gantry motion and,the flat panel detector provide unparalleled performance for low-dose imaging,and automated optical and microCT integration. The stable revolving animal,platform table rotates 360° to acquire full 3D data. Multiple animals can be,scanned simultaneously while maintaining an average dose per scan at about,13mGy, with a scanning and reconstruction time of less than a minute. Optical,and microCT modalities can also operate independently.

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RAS-4 Rodent Anesthesia System

The Xenogen XGI-8 Gas Anesthesia System is designed to work with the IVIS Imaging System, a technology from Xenogen that allows researchers to use r eal-time in vivo imaging to monitor and record cellular and genetic activity within a living organism.

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Software Downloads

Technical Note

Adaptive Fluorescence Background Subtraction

Adaptive Fluorescence Background Subtraction Pre-clinical in vivo imaging technical note for IVIS Imaging Systems. Instrument background occurs when excitation light leaks through the emission filter. This occurs more frequently when the excitation and emission filters are narrowly separated. The ring you see is a result of non specific light reflecting off of the stage at an incident angle and passing through the filter causing what appears as leakage around the edges.

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AutoExposure

Auto-exposure technical note for IVIS pre-clinical imaging systems

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Background ROI

Subtracting Background ROI from a Sequence

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DLIT 1 Setup

DLIT setup and acquisition IVIS pre-clinical imaging systems. Bioluminescence Tomography or Diffuse Light Imaging Tomography (DLIT) utilizes the data obtained from a filtered 2D bioluminescent sequence in combination with a surface topography to represent the bioluminescent source in a 3D space. Utilizing DLIT, you can determine the depth of sources in your animal and calculate the absolute intensity of that source.

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DLIT 3 Reconstruction

DLIT 3 Reconstruction technical note for IVIS Spectrum imaging systems

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Determine Saturation

Determine Saturation for IVIS imaging systems - technical note

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FLIT 1 Setup

FLIT - Fluorescence Tomography – Setup and Sequence Acquisition. Fluorescence Imaging Tomography (FLIT) utilizes the data obtained from a 2D transillumination fluorescence sequence in combination with a surface topography to reconstruct a fluorescent source in a 3D space. Utilizing FLIT, you can determine the depth of sources in your animal and calculate the absolute intensity of that source at depth.

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FLIT 3 Reconstruction

Fluorescence Tomography – Source Reconstruction and Analysis - FLIT Reconstruction

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High Resolution Images

Acquisition of High Resolution Images. This quick reference guide is for those researchers who wish to perform analysis that requires high resolution including in vitro studies when one may want to discern aspects about cell layers, ex vivo tissue imaging, or imaging of tissue slices. You will not need this resolution in most in vivo studies.

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Image Overlay 2D

Not only is it possible to load multiple images as a group, for example multiple days of a longitudinal study, but it is also possible to load multiple images and Overlay them i.e. bioluminescent tumor with fluorescent targeted drug acquired in two separate images.

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Image Overlay 3D

It is possible to copy 3D sources (voxels) from one 3D reconstruction into another. For example, superimposing DLIT or FLIT signals is easy. However, the two combined sources must be based upon the same surface topography to produce meaningful information. Therefore it is imperative that the mouse remain completely still between acquisition of the DLIT and FLIT images.

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Kinetic Analysis of Bioluminescent Sources

Acquiring the most accurate quantitation of your bioluminescent sources requires a close understanding of the underlying kinetics involved in producing and capturing the detected light. After injection, the substrate for your bioluminescent probe will di

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Loading Groups of Images

For many studies, it may be desirable to open a group of images together, for example, analyzing multiple days of longitudinal study side by side using the same scale.

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Spectral Unmixing

This guide will walk you through the steps of manually entering your sequences for the spectral unmixing procedure. The Living Image 4.3.1 software version includes an Autoexposure setting and an Imaging Wizard. For questions on how to use these two features please see the respective quick reference guide associated with these workflows. You can also find information pertaining to the use of these features in the Spectral Unmixing Wizard Setup reference guide. These features are designed to make setting up your sequences as easy as possible and we highly recommend that you take advantage of them when performing these steps.

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Subject ROI

Subject ROI using IVIS imaging systems

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Transillumination 1 Setup

Transillumination is a 2D fluorescence imaging technique that utilizes an excitation light source located below the stage. Transillumination is superior to epi-illumination at detection of red-shifted, deep tissue fluorescent sources due to the transilluminator’s concentrated delivery of excitation light into the subject via a 2 mm beam and lower autofluorescence levels attained due to the position of the animal in relation to the excitation light.

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Transillumination 2 Raster Scan

In order to facilitate faster transillumination imaging, with Living Image 4, we have incorporated raster scanning capabilities. With raster scanning, the shutter remains open as the transillumination excitation source moves underneath the animal. This results in a single image and faster imaging times.,Note: This Transillumination Fluorescence – Raster Scan Tech Note was designed as a supplement to Transillumination Fluorescence Tech Note 14a. For information about setup of your 2D transillumination fluorescence sequences, please first consult that tech note.

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Transillumination 3 Normalized

Normalized transmission fluorescence is a technique that allows us to subtract background light leakage through thin tissue from transillumination images utilizing an extra image captured with a neutral density (ND) filter. The ND filter dampens the intensity of the halogen lamp to 1/100th of the source intensity but does not filter out specific wavelengths. Light of all wavelengths is allowed to pass through the animal and the image is collected with the emission filter of your choice.

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Working with Image Math

Working with Image Math. Image Math is a rudimentary but effective method for Spectrum and Lumina users to subtract background from images without performing Spectral Unmixing.

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