PerkinElmer
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Harmony High-Content Imaging and Analysis Software

Easily quantify complex cellular phenotypes with Harmony® high-content analysis software. Harmony software is designed for PerkinElmer high-content screening systems.

Part Number
Version Number
Model Compatible With
HH17000010
4.9
Operetta CLS AND Opera Phenix
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HH17000001
4.8
Operetta
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HH17000012
5.0
Opera Phenix Plus
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Overview

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

One high-content analysis software for all your applications

  • Phenotypic Screening
    • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
    • Easily train the software to recognize relevant phenotypes
    • Quantify even subtle phenotypic differences
  • Live Cell Imaging
    • Follow phenotypic changes over time
    • Accurately quantify label-free live cell images
    • Track individual cells over time or analyze wound-healing assays
    • Analyze cell signaling dynamics using e.g. FRET based assays
    • Perform fast response assays such as calcium flux or cardiomyocyte beat rate with imaging frame rate of up to 100fps.
    • Set-up "dispense and read" assays on the Opera Phenix Plus system with liquid handling module
    • Enhance your live-cell assay capabilities through improved time-series flexibility
  • 3D Cell Models
    • Speed up 3D image acquisition by targeted imaging of microtissues
    • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
    • Measure morphologies and volumes in 3D, count nuclei within spheroids
    • Enhance Maximum Intensity Projection Analysis using PlaneMap
    • One software for image acquisition, 3D visualization and analysis
  • Rare Cell Phenotypes
    • Quickly capture your rare cell phenotypes with PreciScan
    • Pre-screen at low resolution and acquire objects of interest at high resolution
    • Shorten your image acquisition times
    • Reduce your data volume
  • Routine Assays
    • Utilize more than 30 ready-made-solutions for standard assays
    • Ensure repeatability by running established protocols

One high-content analysis software for all users

  • New Users
    • Get quantification for your cell images
    • Easy to learn – even for users with little microscopy or programming knowledge
    • Get started with 30 ready-made solutions
    • Logical workflow for easy control of all aspects of image acquisition and analysis
    • Simple building blocks to assemble image analysis sequences (step-by-step)
  • Expert Users
    • Quantify difficult phenotypes with advanced texture and morphology readouts
    • Utilize advanced building blocks to enable e.g. ratiometric FRET imaging
    • Create “stitched” global images from multiple fields of view
    • Examine samples at multiple scales – regions found in the global image can be used for analysis in the original images
  • For Lab Managers
    • Keep up with increasing research needs – analyze larger sample sizes
    • Quickly train new users to set up the instrument and analyze their images
    • Find images, metadata and results quickly via the integrated sortable database
    • View and analyze data from any computer with installation of Harmony software
    • Get application support by image analysis experts

Specifications

Model Compatible With Opera Phenix Plus
Version Number 5.0