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Harmony High-Content Imaging and Analysis Software

Easily quantify complex cellular phenotypes with Harmony® high-content analysis software. Harmony software is designed for PerkinElmer high-content screening systems.

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Part Number
Version Number
Model Compatible With
HH17000010
4.9
Operetta CLS AND Opera Phenix
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HH17000001
4.8
Operetta
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Detail Information

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

One high-content analysis software for all your applications

  • Phenotypic Screening
    • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
    • Easily train the software to recognize relevant phenotypes
    • Quantify even subtle phenotypic differences
  • Live Cell Imaging
    • Follow phenotypic changes over time
    • Accurately quantify label-free live cell images
    • Track individual cells over time or analyze wound-healing assays
    • Analyze cell signaling dynamics using e.g. FRET based assays
  • 3D Cell Models
    • Speed up 3D image acquisition by targeted imaging of microtissues
    • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
    • Measure morphologies and volumes in 3D, count nuclei within spheroids
    • Enhance Maximum Intensity Projection Analysis using PlaneMap
    • One software for image acquisition, 3D visualization and analysis
  • Rare Cell Phenotypes
    • Quickly capture your rare cell phenotypes with PreciScan
    • Pre-screen at low resolution and acquire objects of interest at high resolution
    • Shorten your image acquisition times
    • Reduce your data volume
  • Routine Assays
    • Utilize more than 30 ready-made-solutions for standard assays
    • Ensure repeatability by running established protocols

One high-content analysis software for all users

  • New Users
    • Get quantification for your cell images
    • Easy to learn – even for users with little microscopy or programming knowledge
    • Get started with 30 ready-made solutions
    • Logical workflow for easy control of all aspects of image acquisition and analysis
    • Simple building blocks to assemble image analysis sequences (step-by-step)
  • Expert Users
    • Quantify difficult phenotypes with advanced texture and morphology readouts
    • Utilize advanced building blocks to enable e.g. ratiometric FRET imaging
    • Create “stitched” global images from multiple fields of view
    • Examine samples at multiple scales – regions found in the global image can be used for analysis in the original images
  • For Lab Managers
    • Keep up with increasing research needs – analyze larger sample sizes
    • Quickly train new users to set up the instrument and analyze their images
    • Find images, metadata and results quickly via the integrated sortable database
    • View and analyze data from any computer with installation of Harmony software
    • Get application support by image analysis experts

Specifications

Model Compatible With Operetta CLS AND Opera Phenix
Version Number 4.9
Resources, Events & More
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Application Note

Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.

PDF 2 MB
Measuring FRET using the Opera Phenix High-Content Screening System

Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, including cancer. Therefore, inhibiting protein-protein interactions represents an emerging area in drug design.

PDF 1 MB

Booklet

High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches.

Download this booklet to learn how high-content imaging and analysis enable high-throughput functional and phenotypic assays that can be adapted to a wide range of pathogens; read a series of Featured Publication Notes describing the contribution of high-content analysis in the study of diseases such as ebola, zika, tuberculosis, listeria and malaria and find examples of studies in which a high-content approach has been used in parasitic, viral and bacterial disease research.

PDF 4 MB

Brochure

3D Cell Culture Solutions Brochure

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

PDF 4 MB

Technical Note

How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

PDF 3 MB
Opera Phenix High-Content Screening System Technical Performance: Speed

The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera Phenix system’s Synchrony™ Optics and sCMOS cameras image up to 6x faster, how water immersion objectives combined with binning can reduce exposure time by approx. 20-fold and how image acquisition and analysis time can be reduced by >70% compared to a classical spinning disc confocal system.

PDF 708 KB

Video Tutorials

White Paper

High Content Screening in Three Dimensions

Researchers are increasingly looking to 3D cell cultures, microtissues, and organoids to bridge the gap between 2D cell cultures and in vivo animal models. This whitepaper documents a streamlined procedure for getting the most information, as quickly as possible, using solutions from PerkinElmer.

PDF 1 MB