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Harmony High Content Imaging and Analysis Software

Easily quantify complex cellular phenotypes with Harmony high-content analysis software. Harmony software is designed for Operetta, Operetta CLS and Opera Phenix high-content screening systems.

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Part Number
HH17000001
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Overview

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

 

One high-content analysis software for all your applications

  • Phenotypic Screening
    • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
    • Easily train the software to recognize relevant phenotypes
    • Quantify even subtle phenotypic differences
  • Live Cell Imaging
    • Follow phenotypic changes over time
    • Accurately quantify label-free live cell images
    • Track individual cells over time or analyze wound-healing assays
    • Analyze cell signaling dynamics using e.g. FRET based assays
  • 3D Cell Models
    • Speed up 3D image acquisition by targeted imaging of microtissues
    • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
    • Measure morphologies and volumes in 3D, count nuclei within spheroids
    • Enhance Maximum Intensity Projection Analysis using PlaneMap
    • One software for image acquisition, 3D visualization and analysis
  • Rare Cell Phenotypes
    • Quickly capture your rare cell phenotypes with PreciScan
    • Pre-screen at low resolution and acquire objects of interest at high resolution
    • Shorten your image acquisition times
    • Reduce your data volume
  • Routine Assays
    • Utilize more than 30 ready-made-solutions for standard assays
    • Ensure repeatability by running established protocols
 

One high-content analysis software for all users

  • New Users
    • Get quantification for your cell images
    • Easy to learn – even for users with little microscopy or programming knowledge
    • Get started with 30 ready-made solutions
    • Logical workflow for easy control of all aspects of image acquisition and analysis
    • Simple building blocks to assemble image analysis sequences (step-by-step)
  • Expert Users
    • Quantify difficult phenotypes with advanced texture and morphology readouts
    • Utilize advanced building blocks to enable e.g. ratiometric FRET imaging
    • Create “stitched” global images from multiple fields of view
    • Examine samples at multiple scales – regions found in the global image can be used for analysis in the original images
  • For Lab Managers
    • Keep up with increasing research needs – analyze larger sample sizes
    • Quickly train new users to set up the instrument and analyze their images
    • Find images, metadata and results quickly via the integrated sortable database
    • View and analyze data from any computer with installation of Harmony software
    • Get application support by image analysis experts

Resources, Events & More

All (32)
Resource Type File Name File Format
Brochure 3D Cell Culture Solutions Brochure PDF  4 MB
Application Note 3D Analysis of Cell Invasion using Operetta PDF  1 MB
Application Note A Multiparametric Live-Cell Cytotoxicity Analysis using the Operetta PDF  1 MB
Application Note Analysis of Stem Cell Differentiation using Operetta PDF  1 MB
Application Note Automated Single Cell Tracking using Operetta PDF  2 MB
Application Note Comparison of HCA ImagAmp and Conventional Indirect Immunofluorescence for High-Content Analysis Applications PDF  508 KB
Application Note Cytotoxicity Studies on 3D Primary Liver Microtissues PDF  2 MB
Application Note Cytotoxicity Studies on Live Primary Human Hepatocytes using Operetta PDF  2 MB
Application Note Determination of Cell Cycle Phase using the Operetta PDF  618 KB
Application Note Image-based Quantification of PKC Activation using Operetta PDF  827 KB
Application Note Image-based Quantification of the Huntingtin Protein using the Operetta PDF  833 KB
Application Note Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System PDF  2 MB
Application Note Measuring FRET using the Opera Phenix High-Content Screening System PDF  1 MB
Application Note Quantitative Analysis of 3D Microtissue Growth and Biomarker Intensity PDF  916 KB
Application Note Quantitative Analysis of Embryoid Bodies Using the Operetta System PDF  921 KB
Application Note Screening Epigenetic HDAC Inhibitors – A Workflow Comprising HTS and HCS PDF  1 MB
Application Note Signal Amplification Strategies for Rapid and Sensitive High Content Analysis PDF  776 KB
Application Note Studying Kidney Injury using Operetta PDF  1 MB
Application Note Studying NfkB signaling dynamics using Operetta PDF  864 KB
Application Note Visualization of Cathepsin Activity using Operetta and ProSense 680 PDF  1 MB
Technical Note Improved 3D Imaging with Opera Phenix HCS System PDF  1 MB
Technical Note Workflows for In-depth High Content Analysis PDF  1 MB
Poster Automation of both sample preparation and analysis of a cell migration assay PDF  911 KB
Poster FRET-based Quantification of Protein-Protein Interactions using the Opera Phenix HCS System PDF  808 KB
Poster Image-based quantification of HCS cell-based assays using the Operetta/Harmony imaging platform PDF  867 KB
Poster Pushing the Boundaries of 3D Microtissue Analysis using High Content Imaging PDF  990 KB
Poster Screening HDAC Inhibitors – A Workflow Comprising High Throughput And High Content Screening PDF  720 KB
Poster Studying kidney injury using the Operetta High Content Imaging System PDF  454 KB
Case Study Inhibition of MicroRNA Sensitizes 3D Breast Cancer Microtissues to Radiation Therapy PDF  786 KB
Link For comprehensive information about our products, technologies and software for high-content screening and analysis, please visit our microsite Link
Technical Note Opera Phenix High-Content Screening System Technical Performance: Speed Link
Video Tutorials Harmony video tutorial series Link