check quantity

FMT 4000 In Vivo Imaging System

The FMT 4000 fluorescence tomography imaging system provides the greatest utility of the FMT Systems with the ability to quantitate up to four fluorophores simultaneously. It comes with four excitation laser channels (635, 680, 750, & 790 nm).

Please enter valid quantity

Please login to add favorites


Part Number
Buy Now

You successfully added item(s) to your cart


FMT for Fluoresence Tomography: 3 Steps to Generate Fluoresence Tomographic Data in vivo

Quick to learn. Fast to image. Easy to quantify.

STEP 1: FMT Data Generation

  • Transillumination: FMT transillumination of animal using powerful 80 mW lasers
  • Creating Baseline Absorption Profiles: Baseline absorption profiles of each mouse's biological composition are measured by a laser-light raster scan. Paired absorption and fluorescence data maps generated from 10,000 - 100,000 source detector laser projections.

FMT laser-driven transillumination generates paired absorption and fluorescence data maps throughout the animal.

The anesthetized mouse is comfortably placed in PerkinElmer's portable Animal Imaging Cassette. Imaging sessions are rapid (2-3 minutes per animal), animal handling is simple and the mouse remains stable and immobilized for consistent, repetitive imaging results

STEP 2: FMT Normalization

  • Normalized fluorescence measurements generated by processing all paired absorption and fluorescence acquisition data.
  • FMT minimizes the distorting effects of in vivo tissue heterogeneity, taking into account surface boundaries, tissue spectral characteristics and wave-guiding effects to correct and normalize each fluorescent measurement.
  • Data normalized by FMT algorithmic models of photon transport in tissue.

Insert the Animal Imaging Cassette into the FMT 2500. Easily position the anesthetized animal in the Imaging Cassette within the heated imaging chamber. Gas anesthesia is maintained.

STEP 3: FMT Reconstruction

  • Fluorescence quantified to the picomole at each point in the subject including deep tissue targets
  • Fluorescence measurements calculated throughout the user-selected regions of Interest (ROI)
  • Data provided in universal formats for analysis (including DICOM), database building, and decision-making
  • Easily create animated movies including dynamic presentation of quantification data for presentation
  • Data and images easily exported for multi-modality image fusion
  • Quantification is performed on all animals at any depth with equal accuracy and without the need for an artificial image atlas for referential quantification.

Whatever your needs, PerkinElmer has a quantitative FMT platform to provide deeper insights to your biological questions. Select the FMT 1500 for quantitative imaging in individual laboratories, in smaller facilities or for single applications. The 4-channel FMT 2500 LX is available for laboratories requiring a broader range of applications and extensive multi-modality capabilities.

Disclaimer:  For research use only. Not for use in diagnostic procedures.


Imaging Modality Optical Imaging
Optical Imaging Classification Fluorescence Imaging
Portable No
Product Brand Name FMT
Research Areas In Vivo Research

Resources, Events & More

All (31)
Resource Type File Name File Format
Brochure FMT Fluorescence Tomography In Vivo Imaging Systems PDF  1 MB
Product Note RAS-4 Rodent Anesthesia System PDF  562 KB
Application Note Gastric Emptying with GastroSense 750 PDF  1 MB
Application Note Guide to the Use of the Multispecies Imaging Module (MSIM) for Imaging Rats on the FMT Imaging System PDF  1 MB
Application Note Imaging Hepatocellular Liver Injury using NIR-labeled Annexin V PDF  3 MB
Application Note In Vivo Imaging of Atherosclerosis Disease Using FMT PDF  1 MB
Application Note In Vivo Non-Invasive Method to Determine Glomerular Filtration Rate (GFR) PDF  2 MB
Application Note Multiplex 2D Imaging of NIR Molecular Imaging Agents on the IVIS SpectrumCT and FMT 4000 PDF  4 MB
Application Note NIR Fluorescent Cell Labeling for In Vivo Cell Tracking (VivoTrack 680) PDF  2 MB
Application Note Noninvasive In Vivo Quantitation of Asthma Severity Using FMT PDF  488 KB
Technical Note Fluorescence Molecular Tomography (FMTTM) Imaging Techniques PDF  2 MB
Poster A Hybrid Inversion Technique for Extremely Rapid Fluorescence Molecular Tomography Reconstructions PDF  422 KB
Poster A Near-Infrared Fluorescent Transferrin Agent for Quantitative Imaging of Transferrin Receptor Expression in Tumor Xenografts PDF  2 MB
Poster A Technique for Reducing Forward-Model-Induced Artifacts in Fluorescence Molecular Tomography Reconstructions PDF  237 KB
Poster A novel NIR dye for in vivo temporal tracking of labeled macrophages to sites of acute inflammation PDF  689 KB
Poster Combined efficacy & toxicity imaging following acute 5-FU treatment of HT-29 tumor xenografts PDF  1 MB
Poster Fluorescence tomographic imaging of efficacy & toxicity following acute 5-FU treatment of HT29 cells PDF  2 MB
Poster Imaging and quantification of bombesin receptor expression in vivo PDF  1 MB
Poster Imaging of Cathepsin K activity in rodent models of bone turnover and soft tissue calcification PDF  966 KB
Poster In Vivo NIR Imaging and Quantification of Glomerular Filtration Rate (GFR) in Mice PDF  2 MB
Poster In vivo imaging and quantification of bacterial infection using a new red fluorescently labeled agent PDF  1 MB
Poster Molecular imaging of tumor energy metabolism as an early indicator of anti-cancer drug efficacy in small animal models PDF  1023 KB
Poster Quantitative assessment of folate receptor expression by FMT imaging with a NIR fluorescent folate agent PDF  1018 KB
Poster Reduction of excitation source coupling artifacts in diffuse fluorescence tomography reconstructions PDF  749 KB
Poster Targeted in vivo imaging of tumor vasculature using a near infra-red labeled tomato lectin agent PDF  1007 KB
Citations Library View peer reviewed publications using PerkinElmer's In Vivo Imaging systems Link
Software Downloads In Vivo Imaging Software Downloads Link
Video Article JoVE Video Articles Link
Webinars A Novel Non-Invasive NIR Imaging Agent (GFR-Vivo 680) for Measurement of Glomerular Filtration Rate in Mice - View On Demand Link
Webinars View our complete list of in vivo webinars available on demand Link
Webinars In vivo imaging of drug-induced liver injury using NIR fluorescent imaging agents - View On Demand Link