Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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This fluorescein labeled analog of deoxycytidine triphosphate (dCTP) is useful for labeling with a variety of methods including nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. Labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
Fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerizes. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.