Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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NEN brand fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerizes. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used. Some analogs demonstrate variations in relative performance depending upon nucleotide and fluorophore selected due to enzyme preferences. Labeled probes may be used in applications including (but not limited to) chromosome mapping. These analogs are intended to be detected directly by their fluorescence properties.