PerkinElmer
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FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit, 100 Assay Points

This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.

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Part Number Unit Size List Price Your Price Quantity
AL348HV 100 assay points 675.00 USD
AL348C 500 assay points 1803.00 USD
AL348F 5,000 assay points 10300.00 USD
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Overview

This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

These kits use IgG Fc AlphaLISA® Acceptor beads and Streptavidin-coated Donor beads to capture biotinylated human FCGR3A in a competition assay. Donor beads and Acceptor beads come into proximity through IgG Fc fragment binding to FCGR3A. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

The Fc-Gamma Receptors (FCGRs) are members of immunoglobulin superfamily and play a critical role in the function of therapeutic antibodies. FCGRs are divided into three classes and FCGR3 (CD16) is expressed as two distinct forms (FCGR3A and FCGR3B) encoded by two different highly homologous genes in a cell type specific manner. FCGR3A is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), mast cell degranulation and clearance of immune complexes. In humans, a single nucleotide polymorphism creates two isoforms: high binding (176Val/V158) and low binding (176Phe/F158) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. FCGR3A has been considered as an important therapeutic target. This AlphaLISA assay can be used to determine the binding activity of human IgG Fc fragment to human FCGR3A and also can be used to study how other antibodies bind to FCGR3A by competition assay.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target FCGR3A
Assay Target Class Fc-receptor
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Biologics/Bioprocess
Unit Size 100 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Fc-Receptor Binding ADCC Assays Utilizing AlphaLISA: Characterization of hIgGs and FcγRIIIa PDF  1 MB
Data Sheet AlphaLISA FCGR3A 176Val Binding Kit AL348 PDF  604 KB
Event Society of Laboratory Automation & Screening (SLAS)