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DELFIA® EuTDA Cytotoxicity Reagents

DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and antibody-dependent cell cytotoxicity (ADCC). This kit has the same assay principle as a chromium release assay, with target cells loaded with a BATDA ligand instead of 51Cr. There is no requirement for specially-engineered cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

DELFIA time-resolved fluorescence cell cytotoxicity assays offer a non-radioactive method to Chromium 51, for use in cell-mediated cytotoxicity and antibody-dependent cell cytotoxicity (ADCC) studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Reagents for the assay are also available separately:

  • Lysis buffer (30 mL), #4005-0010
  • DELFIA Eu-Solution (200 mL), #C135-100
  • DELFIA BATDA labeling reagent (50 µL), #C136-100

Selected references:

  1. Lazar, G. A. et al. Engineered antibody Fc variants with enhanced effector function. PNAS 103, 4005–4010 (2006).
  2. Tsai, C.-Y. et al. Type I IFNs and IL-18 Regulate the Antiviral Response of Primary Human γδ T Cells against Dendritic Cells Infected with Dengue Virus. The Journal of Immunology 194, 3890–3900 (2015).
  3. Mehta, R. S., Chen, X., Antony, J., Boyiadzis, M. & Szabolcs, P. Generating Peripheral Blood Derived Lymphocytes Reacting Against Autologous Primary AML Blasts. J Immunother 39, 71–80 (2016).
  4. Snyder, K. M. et al. Expression of a Recombinant High Affinity IgG Fc Receptor by Engineered NK Cells as a Docking Platform for Therapeutic mAbs to Target Cancer Cells. Front Immunol 9, (2018).
  5. Yu, T., Qiao, C., Lv, M. & Tang, L. Novel anti-CD38 humanized mAb SG003 possessed enhanced cytotoxicity in lymphoma than Daratumumab via antibody-dependent cell-mediated cytotoxicity. BMC Biotechnology 19, 28 (2019).

Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Experimental Type In vitro
Product Brand Name DELFIA
Shipping Condition Blue Ice
Unit Size 1 Kit
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Brochure

Drug Discovery Screening Solutions Brochure

Info on PerkinElmer's complete solutions for high-throughput and phenotypic screening.

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Manual

DELFIA Cell Cytotoxicity Manual

This product is intended for loading of cells to be used in short term cytotoxicity tests performed utilizing time-resolved fluorometry in the detection.

PDF 55 KB