DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and ADCC.
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DELFIA® cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
Reagents for the assay are also available separately: 4005-0010 Lysis buffer (30 mL), C135-100 DELFIA Eu-Solution (200mL), C136-100 DELFIA BATDA labeling reagent (50µL).
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Quantity in a Package Amount||960.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||1 Kit|
Discover PerkinElmer's simplified, gentle cell-labelling method for non-radioactive cytotoxicity assays
Info on PerkinElmer's complete solutions for high-throughput and phenotypic screening.
This product is intended for loading of cells to be used in short term cytotoxicity tests performed utilizing time-resolved fluorometry in the detection.