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LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody, 10 µg

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.

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Part Number Unit Size List Price Your Price Quantity
TRF0404-D 10 µg 3484.00 USD
TRF0404-M 100 µg 8700.00 USD
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The LANCE® Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) antibody was used for the development and optimization of a SIRT1 deacetylation assay using a biotinylated Histone H3 (1-21) peptide acetylated at lysine 4 as substrate. A technical note describing the assay is available in our product literature.


  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.

Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.


Antibody Conjugates Anti-H3K4
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Acetylation,Methylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 10 µg